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Alexa Fluor® 488 anti-mouse IL-17A Antibody
产品名称：
Alexa Fluor® 488 anti-mouse IL-17A Antibody
产品类别：
抗体
产品编号：
506909
产品应用：
506909
[价格]
规格 价格 库存
25µg ￥ 1736 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed, recombinant mouse IL-17A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

ELISA Capture3,4 and ELISPOT Capture5: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 576009) as the standard.
Flow Cytometry2-4,7,8,11,12: The TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17-producing cells within mixed cell populations.
Neutralization6,9: The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (Cat. No. 506906).

Application References

(PubMed link indicates BioLegend citation)
  1. Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611.
  2. Schubert D, et al. 2004. J. Immunol. 172:4503. (ICFC)
  3. Infante-Duarte C, et al. 2000. J. Immunol. 165:6107. (ICFC, ELISA Capture)
  4. Harrington LE, et al. 2005. Nature Immunol. doi:10.1038/ni1254. (ICFC, ELISA Capture)
  5. Nekrasova T, et al. 2005. J. Immunol. 175:2734. (ELISPOT Capture)
  6. Yen D, et al. 2006. J. Clin. Invest. 116:1310. (Neut)
  7. Ehirchiou D, et al. 2007. J. Exp. Med. 204:1519. (ICFC)
  8. Kang SG, et al. 2007. J. Immunol. 179:3724. (ICFC)
  9. Smith E, et al. 2008. J. Immunol. 181:1357. (Neut) PubMed
  10. Neufert C, et al. 2007. Eur. J. Immunol. 37:1809. PubMed
  11. Wang C, et al. 2009. Mucosal Immunol 2:173. (ICFC) PubMed
  12. Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (ICFC) PubMed
  13. Kivis?kk P, et al. 2009. Ann. Neurol. 65:457. PubMed
  14. Cooney LA, et al. 2011. J. Immunol. 187:4440. PubMed
  15. Ma Y, et al. 2012. PLoS One. 7:e40763. PubMed
  16. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Product Citations
  1. Gültner S, et al. 2010. Eur J Immunol. 40:3403. PubMed
  2. Han SJ et al. 2017. Immunity. 47(6):1154-1168 . PubMed
  3. Wilfahrt D, et al. 2021. Elife. 10:. PubMed
  4. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  5. Kim Y, et al. 2015. PLoS One. 10:120294. PubMed
  6. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
  7. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  8. Meng KP, et al. 2020. J Exp Med. 217:00:00. PubMed
  9. Obregón-Henao A, et al. 2013. PLoS One. 8:80669. PubMed
  10. Liu S, et al. 2017. J Pharmacol Sci. 10.1016/j.jphs.2017.02.001. PubMed
  11. Maston LD, et al. 2017. Am J Physiol Lung Cell Mol Physiol. 312:L609. PubMed
  12. Snyder LM, et al. 2022. Immunohorizons. 6:660. PubMed
  13. Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
  14. Kim E, et al. 2022. STAR Protoc. 3:101366. PubMed
  15. Lu M, et al. 2021. J Virol. 95:e0059221. PubMed
  16. Stacy A, et al. 2021. Cell. 184(3):615-627.e17. PubMed
  17. Dudeck J, et al. 2019. J Allergy Clin Immunol. 143:1849. PubMed
  18. Cheng HW, et al. 2022. Nat Commun. 13:2027. PubMed
  19. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  20. Lee Y, et al. 2012. Nat Immunol. 13:991. PubMed
  21. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed
RRID
AB_536011 (BioLegend Cat. No. 506909) AB_536012 (BioLegend Cat. No. 506910)

Antigen Details

Structure
Cytokine; dimer; 15 kD (Mammalian).
Bioactivity
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources
CD4+ memory T cells
Cell Targets
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors
IL-17R (CD217)
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006. Nat. Rev. Immunol. 6:329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237:123.

Gene ID
16171 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

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