Anti-Tau, 15-25 Antibody (Previously Covance catal-上海雅吉生物科技有限公司

规格	价格	库存
500µL	￥ 5866	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Preparation

Ascites

Concentration

The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.

Storage & Handling

Store at -20°C or below. Upon initial thawing, apportion into working aliquots and store at -20°C or below. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Do not store in frost-free freezers.

Application

WB - Quality tested
IHC-Other - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, a dilution range of 1:500-1:1000 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

TAU-13 is a mouse monoclonal antibody that binds to human tau and is able to stain brain tissue early in Alzheimer’s disease. TAU-13 is specific for human tau; it does not react with bovine, murine or rat tau. Preliminary assignment of its epitope indicates that it maps to amino acid residues 15-25 on the longest isoform of human tau.

This antibody clone has been reported for use on IHC of 4% PFA-fixed free floating sections¹.

Application References (PubMed link indicates BioLegend citation)

Bi M, et al. 2011. PLoS ONE. 6:12. (IHC-other)
García-Sierra F, et al. 2003. J Alzheimers Dis. 5:65–77. (IHC-other)

Product Citations

Han J, et al. 2021. Theranostics. 11:8855. PubMed
Crotti A, et al. 2019. Sci Rep. 9:9477. PubMed
Liu P, et al. 2019. Acta Neuropathol Commun. 7:111. PubMed
Busche MA, et al. 2019. Nat Neurosci. 22:57. PubMed
Pickett EK, et al. 2019. Cell Rep. 29:3592. PubMed
Zhu B, et al. 2022. Mol Neurodegener. 17:58. PubMed
McCreedy DA, et al. 2021. Front Cell Neurosci. 15:684792. PubMed
Zhao X, et al. 2016. Nat Med. 22:1268-1276. PubMed
Liu P, et al. 2020. Sci Rep. 10:3869. PubMed
Rauch JN, et al. 2020. Nature. 580:381. PubMed
Fu H, et al. 2016. PLoS One. 11: 0159463. PubMed
Rasmussen J, et al. 2021. Brain Pathol. :e13018. PubMed
Nogueras-Ortiz C, et al. 2014. Front Neurosci. 7:277. PubMed

RRID

AB_2565341 (BioLegend Cat. No. 835201)

Antigen Details

Structure

Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.

Distribution

Tissue distribution: Central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: Cytoskeleton, nucleus, plasma membrane, and cytosol.

Function

Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Interaction

Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, casein kinase I isoform delta, serine/threonine-protein kinase Sgk1, Laforin, and alpha-synuclein.

Biology Area

Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation

Molecular Family

Tau

Antigen References