Purified anti-p63 (TA) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1904	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Full-length recombinant human p63 (TA) protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

WB - Quality tested
ICC, IP - Verified

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1 ?g/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 ?g/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Epitope binding of TAp63-4.1 has been mapped to the LSDPxW motif at the C-terminal region of the p63 transactivation (TA) domain (Nekulova M, et al. 2013. Virchows Arch. 463:415). Because this sequence is absent in the p63 (?N isoform) and the p63 paralog p73, this antibody is not predicted to react with either protein. Lack of p63 (?N) reactivity was confirmed during product development testing.

This clone was ICC tested on Raji cells fixed with 4% PFA and permeabilized with either Triton X-100 or methanol. Both methods were compatible with p63 (TA) staining.

This clone is predicted to recognize all p63 (TA) isoforms.

This clone is predicted to recognize mouse p63 (TA) due to complete sequence conservation of the LSDPxW epitope between human and mouse p63 (TA) orthologs.

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