Anti-α-Synuclein Antibody (Previously Covanc
- 产品名称:
- Anti-α-Synuclein Antibody (Previously Covanc
- 产品类别:
- 抗体
- 产品编号:
- 838202
- 产品应用:
- 838202
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Syn 204 was raised using recombinant alpha synuclein and recognizes residues 1-130 of alpha synuclein.
- Preparation
- Ascites
- Concentration
- The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
- Storage & Handling
- Store at -20°C. It is suggested that the total volume be divided into useable aliquots upon initial thaw.
- Application
-
IHC-P - Quality tested
WB - Verified
ICC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:1000 - 1:5000 is suggested.?For Western blotting, the suggested use of this reagent is 1:1000 - 1:2000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC-P), and immunocytochemistry (ICC).
- Application References
(PubMed link indicates BioLegend citation) -
- Yazawa I, et al. 2005. Neuron. 45:847
- Giasson BI, et al. 2000. J Neurosci Res. 59(4):528
- Follenzi A, et al. 2008. J. Clin. Invest. 2:777. (WB)
- Galvin JE, et al. 2000. Am. J. Pathol. 2:361. (IHC-P)
- Luk KC, et al. 2014. Nat. Protoc. 9:2135. (ICC)
- Lippa CF, et al. 2001. Arch. Neurol. 11:1817. (IHC-P)
- Liu Y, et al. 2014. F1000Res. 3:68. (WB)
- RRID
- AB_2734614 (BioLegend Cat. No. 838202) AB_2565390 (BioLegend Cat. No. 838201)