Anti-Synuclein-α/β, 130-140 Antibody (P
- 产品名称:
- Anti-Synuclein-α/β, 130-140 Antibody (P
- 产品类别:
- 抗体
- 产品编号:
- 836602
- 产品应用:
- 836602
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Syn 202 was raised using recombinant alpha and beta synucleins and recognizes residues 130-140 in alpha and beta synuclein.
- Preparation
- Ascites
- Concentration
- The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
- Storage & Handling
- Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
- Application
-
IHC-P -?Quality tested
WB -?Verified
EM, ICC, IP -?Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:500-1:1000 is suggested. For Western blotting, the suggested dilution of this reagent is 1:500-1:1000. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody is effective in immunoblotting (WB), immunoprecipitation (IP), and immunohistochemistry of formalin-fixed paraffin-embedded tissue sections (IHC-P).
- Application References
(PubMed link indicates BioLegend citation) -
- Nibe K,?et al. 2009.?Vet. Pathol.?46(3):474-83.?(IHC)
- Giasson BI,?et al. 2000.?J. Neurosci. Res.?59(4):528-33.
- Mazzulli JR, et. al. 2011. Cell 146:37. (WB)
- Giasson BI, et. al. 1999. J. Biol. Chem. 274:7619. (EM)
- Giasson BI, et. al. 2002. Neuron. 34:521. (ICC)
- Usenovic M, et. al. 2012. J. Neurosci. 32:4240. (WB) PubMed
- Morrison GJ, et. al. 2012. PLoS One 7:e43288. (IP)
- Knight AL, et. al. 2014. Cell Metab. 20:145. (WB) PubMed
- Product Citations
-
- Knight A, et al. 2014. Cell Metab. 20:145-157. PubMed
- Usenovic M, et al. 2012. J Neurosci. 32:4240-4246. PubMed
- Stoyka LE, et al. 2021. eneuro. 8(3):. PubMed
- RRID
- AB_2734606 (BioLegend Cat. No. 836602) AB_2565365 (BioLegend Cat. No. 836601)