Purified anti-Synaptophysin Antibody
- 产品名称：
- Purified anti-Synaptophysin Antibody
- 产品类别：
- 抗体
- 产品编号：
- 837103
- 产品应用：
- 837103
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was raised against a crude synaptic preparation from post mortem human brain.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IHC-P - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 ?g per mL.?For immunocytochemistry, a concentration range of 1.0 - 10 μg/mL?is recommended.?For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0?- 10??g/mL?for chromogenic staining and 0.5?- 2.0??g/mL?for fluorescent staining is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
SP17 reacts with native, denatured and recombinant synaptic vesicle protein synaptophysin expressed in CHO cells. The antibody is particularly valuable for immunocytochemical studies of the brain due to its stability in formalin sections.?
- Application References
(PubMed link indicates BioLegend citation) -
- Frigo DE and McDonnell DP. 2008. Mol. Cancer Ther. 3:659. (WB) PubMed
- Wolf AB, et al. 2012. J Alzheimers Dis. 1:217. (ELISA) PubMed
- Wolf A, et al. 2012. J Alzheimers Dis. 32:217-232. (ELISA) PubMed
- McDonnell D 2008. Mol Cancer Ther. 7:659-669. (WB) PubMed
- Product Citations
-
- Yasom S, et al. 2022. FASEB Bioadv. 4:408. PubMed
- RRID
- AB_2783410 (BioLegend Cat. No. 837103) AB_2783411 (BioLegend Cat. No. 837104)
- Structure
- Synaptophysin is a 313 amino acid protein with an apparent molecular mass of 34 kD.
- Distribution
-
Tissue distribution: Brain, pancreas, adipose and soft tissue, gastrointestinal tract, bone marrow, and immune system.
Cellular Distribution: Plasma and synaptic vesicle membrane.
- Function
- Synaptophysin plays a role in synaptic vesicle docking and membrane fusion.
- Interaction
- SRCIN1, also interact with itself to form homohexamer or homotetramer
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Molecular Family
- Presynaptic proteins
- Antigen References
-
- Zhang L, et al. 2018. Medicine (Baltimore). 97(29):e11487
- Moris, et al. 2018. Anticancer res. 38(2):601
- Hami J, et al. 2017. J pediatr Neurosci. 12(3):215
- Gene ID
- 6855 View all products for this Gene ID
- UniProt
- View information about Synaptophysin on UniProt.org
