Purified anti-Synaptobrevin (VAMP) Antibody
- 产品名称：
- Purified anti-Synaptobrevin (VAMP) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 837003
- 产品应用：
- 837003
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was raised against a crude synaptic preparation of post mortem human brain.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified
ELISA, ICC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 ?g per ml.?For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
SP10 reacts with native, denatured and recombinant synaptic vesicle proteins synaptobrevin/VAMP-1 (aa 1-118) and VAMP-2 expressed in a bacterial system (aa 33-96). The antibody is particularly valuable for immunocytochemical studies of the brain due to its stability in formalin sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Sawada K, et al. 2002. Mol Psychiatry 7:484-92. (WB, IHC-P, ELISA)
- Honer WG, et al. 2002. Cerebral Cortex 12:349-56. (WB, ELISA, ICC)
- Honer WG, et al. 1993. Brain Res. 609:9-20. (ICC)
- Poon VY, et al. 2014. Front. Cell Neurosci. 8:66. (ICC) PubMed
- RRID
- AB_2810717 (BioLegend Cat. No. 837003) AB_2810718 (BioLegend Cat. No. 837004)
- Structure
- Synaptobrevin 1 (VAMP1) is a 118 amino acid protein with an apparent molecular mass of 13kD. Synaptobrevin 2 (VAMP2) is a 116 amino acid protein with an apparent molecular mass of 12.5 kD.
- Distribution
-
Tissue distribution: Nervous system, skeletal muscle and adipose tissue.
Cellular distribution: Synaptic vesicle membrane, cytoplasmic vesicle membrane, and mitochondrion
- Function
- Synaptobrevin is involved in the targeting and/or fusion of transport vesicles to their target membrane.
- Interaction
- VAPA, VAPB, Syntaxin, SNAP25
- Ligand/Receptor
- SNAPs
- Cell Type
- Neurons
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
- Molecular Family
- Neurotransmitter Receptor Trafficking/Endocytosis, Presynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
- Gene ID
- 6843 View all products for this Gene ID
- UniProt
- View information about Synaptobrevin on UniProt.org
