Anti-Synaptobrevin (VAMP) Antibody (Previously Cov-上海雅吉生物科技有限公司

规格	价格	库存
500µL	￥ 6118	1

Product Details

Verified Reactivity

Human, Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

This antibody was raised against a crude synaptic preparation of post mortem human brain.

Preparation

Ascites

Concentration

The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.

Storage & Handling

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.

Application

WB - Quality tested
IHC-P, ELISA, ICC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution of this reagent is 1:1000 - 1:2000. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

SP10 reacts with native, denatured and recombinant synaptic vesicle proteins synaptobrevin/VAMP-1 (aa 1-118) and VAMP-2 expressed in a bacterial system (aa 33-96). The antibody is particularly valuable for immunocytochemical studies of the brain due to its stability in formalin sections.?

Application References (PubMed link indicates BioLegend citation)

Sawada K, et al. 2002. Mol Psychiatry 7:484-92. (WB, IHC-P, ELISA)
Honer WG, et al. 2002. Cerebral Cortex 12:349-56. (WB, ELISA, ICC)
Honer WG, et al. 1993. Brain Res. 609:9-20. (ICC)
Poon VY, et al. 2014. Front. Cell Neurosci. 8:66. (ICC) PubMed

Product Citations

Poon V, et al. 2014. Front Cell Neurosci. 8:66. PubMed

RRID

AB_2565369 (BioLegend Cat. No. 837001)

Antigen Details

Structure: Synaptobrevin 1 (VAMP1) is a 118 amino acid protein with an apparent molecular mass of 13kD. Synaptobrevin 2 (VAMP2) is a 116 amino acid protein with an apparent molecular mass of 12.5 kD.
Biology Area: Cell Biology, Neuroscience, Synaptic Biology
Molecular Family: Presynaptic proteins
Gene ID: 6843 View all products for this Gene ID
UniProt: View information about Synaptobrevin on UniProt.org

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