- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution + 0.1% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
IHC-P -?Quality tested
WB -?Verified
ICC -?Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.25 – 0.5 ?g/ml is suggested. For Western blotting, the suggested use of this reagent is 0.2 – 1.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody reacts with the sequence Ala-Ser-Asp-Tyr-Lys-Ser in position 131-136 of the classic human myelin basic protein. The antibody detects myelin basic protein from most mammalian species. This antibody detects developing and adult myelin, developing oligodedrocytes and distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain sections. The combination of this antibody with clone SMI-91 and/or SMI-94 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.
- Additional Product Notes
Myelin basic protein exists in multiple isoforms. By WB, clone SMI 99 detects mainly?the 21 kD and 28 kD isoforms in the murine brain tissue, and the 21 kD isoform in the human brain tissue.
- Application References
(PubMed link indicates BioLegend citation) -
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- RRID
- AB_2734562 (BioLegend Cat. No. 808403) AB_2564741 (BioLegend Cat. No. 808401) AB_2564742 (BioLegend Cat. No. 808402)
