- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
This antibody reacts with the sequence Ala-Ser-Asp-Tyr-Lys-Ser in position 131-136 of the classic human myelin basic protein. The antibody detects myelin basic protein from most mammalian species. This antibody detects developing and adult myelin, developing oligodedrocytes and distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain sections. The combination of this antibody with clone SMI-91 and/or SMI-94 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.
- Additional Product Notes
Myelin basic protein exists in multiple isoforms. By WB, clone SMI 99 detects mainly?the 21 kD and 28 kD isoforms in the murine brain tissue, and the 21 kD isoform in the human brain tissue.
- Application References
(PubMed link indicates BioLegend citation) -
- Fyffe-Maricich SL, et al.?2011. J. Neurosci.?31(3):843-50.?(IHC, WB)?PubMed
- Mendz GL, et al.?1985. Biochem.?228(1):61-8.
- Kerlero De Rosbo N,?et al.?1984.?Neurochem. Res.?9(10):1359-69.
- Carnegie PR, et al.?1983. J. Neuroimmunol.?5(2):125-34.
- Dowse CA, et al.?1983. J. Neuroimmunol.?5(2):135-44.
- Del Valle L,et al. Cancer Biol Ther. 9:286. (IHC) PubMed
- Kiryu-Seo S, et al.?2010. J. Neurosci.?30(19):6658-66.?(ICC) PubMed
- Samanta J, et al.?2010. Stroke.?41(2):357-62.?(IHC) PubMed
- Werner HB, et al. 2007.?J. Neurosci.?27(29):7717-30.?(WB, ICC) PubMed
- RRID
- AB_2814551 (BioLegend Cat. No. 808407) AB_2814552 (BioLegend Cat. No. 808408)