Purified anti-Myelin CNPase Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1568	1

Product Details

Verified Reactivity

Human, Rat, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

IHC-P - Quality tested
WB -?Verified
IHC-F, ICC, EM, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 1.0 ?g/mL. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 ?g/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissue sections^1,2,4,8?(IHC), immunocytochemistry^5,6 (ICC), and western blot (WB).^3,7

Application References (PubMed link indicates BioLegend citation)

Samanta J,?et al.?2010.?Stroke.?2:357-62. (IHC-F)?
Werner HB,?et al.?2007.?J. Neurosci.?27:7717-7730. (IHC-P)?PubMed
McFerran B,?et al. 1997.?J Cell Sci. 110:2979-2985. (WB)
Trolle C,?et al.?2014.?BMC Neurosci.?15:60. (IHC-F) PubMed
Liyanage VR,?et al.?2013.?Mol. Autism?4:46. (ICC)
G?ttle P, et al. 2015. J. Neurosci. 35:906. (ICC)
Yu C,?et al.?2008.?Glia.?56:877. (WB)
Johnson GC,?et al.?2014.?Pathol.?51:146. (IHC-P) PubMed

Product Citations

Huang S, et al. 2021. STAR Protocols. 2(2):100416. PubMed
Zhao X, et al. 2021. iScience. 24:103485. PubMed
Li AM, et al. 2021. Cereb Cortex. 31:4340. PubMed
Chew LJ, et al. 2020. Cell Reports. 29(10):3173-3186.e7.. PubMed
Shi Y, et al. 2019. J Exp Med. 216:2546. PubMed
Martín–López E, et al. 2018. Brain Struct Funct. 223:4067. PubMed
Stojic A et al. 2018. Glia. 67(3):512-524 . PubMed
Werner H, et al. 2007. J Neurosci. 27:7717-7730. PubMed
Huerga-Gómez A, et al. 2020. Glia. . PubMed
Rahman A et al. 2018. The Journal of comparative neurology. 527(4):797-817 . PubMed
Schwabenland M, et al. 2021. Immunity. . PubMed
Hill RA, et al. 2018. Nat Neurosci. 21:683. PubMed
Jablonska B, et al. 2022. Nat Commun. 13:4771. PubMed
Samanta J, et al. 2010. Stroke. 41:357-362. PubMed

RRID

AB_2728547 (BioLegend Cat. No. 836403) AB_2566639 (BioLegend Cat. No. 836404)

Antigen Details

Structure

Reacts with the 46 kD and 48 kD subunits of the 94 kD myelin CNPase dimer.

Distribution

Tissue distribution: Central and peripheral nervous systems.
Cellular distribution: Cytoskeleton, nucleus, mitochondria, cytosol, and plasma membrane.

Function

CNPase detects developing and adult myelin, and developing oligodendrocytes and Schwann cells. CNPase distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain sections.

Interaction

Detects developing and adult myelin and developing oligodendrocytes and Schwann cells. Distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain sections.

Cell Type

Oligodendrocytes

Biology Area

Cell Biology, Neuroscience, Neuroscience Cell Markers

Molecular Family

Enzymes and Regulators, Phospho-Proteins

Antigen References