Alexa Fluor® 647 anti-Myelin CNPase Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 2226	1

Product Details

Verified Reactivity

Human, Rat, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

IHC-P -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissue sections^1,2,4,8?(IHC), immunocytochemistry^5,6 (ICC), and western blot (WB).^3,7

Additional Product Notes

The reactivity of Alexa Fluor? 647 anti-Myelin CNPase antibody was verified in FFPE mouse and rat brain tissue.

Application References (PubMed link indicates BioLegend citation)

Samanta J,?et al.?2010.?Stroke.?2:357-62. (IHC-F)?
Werner HB,?et al.?2007.?J. Neurosci.?27:7717-7730. (IHC-P)?PubMed
McFerran B,?et al. 1997.?J Cell Sci. 110:2979-2985. (WB)
Trolle C,?et al.?2014.?BMC Neurosci.?15:60. (IHC-F) PubMed
Liyanage VR,?et al.?2013.?Mol. Autism?4:46. (ICC)
G?ttle P, et al. 2015. J. Neurosci. 35:906. (ICC)
Yu C,?et al.?2008.?Glia.?56:877. (WB)
Johnson GC,?et al.?2014.?Pathol.?51:146. (IHC-P) PubMed

RRID

AB_2734604 (BioLegend Cat. No. 836407) AB_2734605 (BioLegend Cat. No. 836408)

Antigen Details

Structure

Reacts with the 46 kD and 48 kD subunits of the 94 kD myelin CNPase dimer.

Distribution

Tissue distribution: Central and peripheral nervous systems.
Cellular distribution: Cytoskeleton, nucleus, mitochondria, cytosol, and plasma membrane.

Function

CNPase detects developing and adult myelin, and developing oligodendrocytes and Schwann cells. CNPase distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain sections.

Interaction

Detects developing and adult myelin and developing oligodendrocytes and Schwann cells. Distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain sections.

Cell Type

Oligodendrocytes

Biology Area

Cell Biology, Neuroscience, Neuroscience Cell Markers

Molecular Family

Enzymes and Regulators, Phospho-Proteins

Antigen References