| 规格 | 价格 | 库存 |
|---|---|---|
| 500µL | ¥ 4872 | 1 |
ICC -?Quality tested
ELISA, WB
The optimal working dilution should be determined for each specific assay condition. The extent of permissible dilution of SMI 62 beyond those recommended for general application depends upon nature and concentration of the antigen examined, species of the antigen, method of fixation and kind of section examined.
? WB: 1:1,000
? ICC: 1:1,000
? ELISA: 1:1,000
This antibody is effective in immunoblotting (WB), immunocytochemistry (ICC), and ELISA.
SMI 62 reacts with polymerized ?-tubulin. The antibody has a preference for recognizing polymerized tubulin in immunocytochemistry. This antibody helps reduce background in cell staining applications caused by unpolymerized tubulin in the cytoplasm. Reaction is observed with cold-stabilized tubulin, premitotic, interphase and mitotic nuclei and flagellar tubulin. SMI 62 reacts with most mammalian species in neuronal and non-neuronal tissue. Green algae and molds also react with SMI 62.
?
Observed MW is at 50 kDa.
This clone shows high reactivity towards other ?–tubulin isotypes.