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Anti-Tubulin β-3, Polymerized (TUBB3) Antibod
产品名称:
Anti-Tubulin β-3, Polymerized (TUBB3) Antibod
产品类别:
抗体
产品编号:
921001
产品应用:
921001
[价格]
规格 价格 库存
500µL ¥ 4872 1

产品详情

Product Details

Verified Reactivity
Mammalian, Green Algae, Molds
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Ascites Fluid (contains 0.01M sodium azide).
Preparation
Ascites
Concentration
The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

ICC -?Quality tested
ELISA, WB

Recommended Usage

The optimal working dilution should be determined for each specific assay condition. The extent of permissible dilution of SMI 62 beyond those recommended for general application depends upon nature and concentration of the antigen examined, species of the antigen, method of fixation and kind of section examined.
? WB: 1:1,000
? ICC: 1:1,000
? ELISA: 1:1,000

Application Notes

This antibody is effective in immunoblotting (WB), immunocytochemistry (ICC), and ELISA.

SMI 62 reacts with polymerized ?-tubulin. The antibody has a preference for recognizing polymerized tubulin in immunocytochemistry. This antibody helps reduce background in cell staining applications caused by unpolymerized tubulin in the cytoplasm. Reaction is observed with cold-stabilized tubulin, premitotic, interphase and mitotic nuclei and flagellar tubulin. SMI 62 reacts with most mammalian species in neuronal and non-neuronal tissue. Green algae and molds also react with SMI 62.
?

Observed MW is at 50 kDa.

This clone shows high reactivity towards other ?–tubulin isotypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Zhu B, et al. 2010. Am J Physiol Lung Cell Mol Physiol. 299: L493. (WB) PubMed
  2. Fortune B, et al. 2008. Invest. Ophthalmol. Vis. Sci. 49: 255.
Product Citations
  1. Zhu B, et al. 2010. Am J Physiol Lung Cell Mol Physiol. 299:L493-501. PubMed
RRID
AB_2565385 (BioLegend Cat. No. 921001)

Antigen Details

Cell Type
Mature Neurons
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microtubules
Gene ID
203068 View all products for this Gene ID
UniProt
View information about Tubulin beta-3 Polymerized on UniProt.org

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