Alexa Fluor® 647 anti-MAP2 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1806	1

Product Details

Verified Reactivity

Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

This monoclonal antibody was raised against Microtubule-Associated Protein 2 (MAP2).

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

IHC-P - Quality tested
3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 ?g/mL?is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for relevant formats) include: immunocytochemistry^5,6, ELISA⁷,?and?WB^1,5.

SMI 52 reacts with microtubule-associated protein 2 (MAP2). This antibody reacts with most mammalian species and with frog tissue. SMI 52 recognizes neuronal cell bodies and dendrites in tissue sections and cell cultures. In immunoblots of brain cytoskeletal preparations, SMI 52 reacts with MAP2a and b, and also with a doublet at 68 kD that may represent MAP2c.

Application References (PubMed link indicates BioLegend citation)

Shi Y, et al. 2017. Cell Death Differ. 24(1):167. (WB)
Shelton MA, et al. 2015. Biol. Psychiatry. 78(6):374. (IHC) PubMed
Wang X, et al. 2015. PLoS One. 10: 0145441. (IHC) PubMed
Kazim SF, et al. 2014. Neuro Dis. 71:110. (IHC)
Cardenas-Aguayo MC, et al. 2013. PLOS ONE. (1):e53596. (ICC, WB) PubMed
Lo Furno D, et al. 2013. J. Cell. Physiol. 228:2109. (ICC)
Gensel J, et al. 2009. J Neurosci. 29:3956-3968. (ELISA) PubMed

Product Citations

Ullah I, et al. 2021. Immunity. 54:2143. PubMed

RRID

AB_2721422 (BioLegend Cat. No. 801806) AB_2721423 (BioLegend Cat. No. 801807)

Antigen Details

Structure

MAP2 is made up of two bands of an apparent molecular weight of approximately 280 kD, referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kD on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternative transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein τ (tau).

Distribution

Cellular distribution: Plasma membrane, cytoskeleton, nucleus, and cytosol.
Tissue distribution: Predominantly in the nervous system

Function

MAP2 encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are involved in microtubule assembly. The products of these genes are neuron-specific cytoskeletal proteins that are enriched in dendrites, which implicate a role in determining and stabilizing dentritic shape during neuron development.

Interaction

MAP2 interacts with microtubules. It has also been reported to interact with KNDC1 via KIND2.

Ligand/Receptor

MAP2 isoforms are expressed in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast τ (tau) is found predominantly in neuronal axons.

Cell Type

Neural Stem Cells

Biology Area

Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells

Molecular Family

Microtubules

Antigen References