Anti-GFAP (Cocktail) (Previously Covance catalog# -上海雅吉生物科技有限公司

规格	价格	库存
100µL	￥ 4340	1

Product Details

Verified Reactivity

Human, Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Ascites Fluid (contains 0.01M sodium azide).

Preparation

Ascites

Concentration

The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.

Storage & Handling

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Application

IHC-P - Quality tested
ICC, WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:500 - 1:1000 is suggested. For western blotting, a dilution range of 1:1000 - 1:2000 is suggested.? For immunocytochemistry, a dilution range of 1:500 - 1:1000 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Antibody is less reactive in mouse tissue in IHC-P.

Multiple protein fragments ranging from 38 to 48 kD have been reported in human CNS lysates resulting from caspase- and calpain-mediated cleavage of GFAP.

Product Citations

Sara Calabretta et al. 2018. Developmental cell. 46(4):426-440 . PubMed
Chen M, et al. 2013. ASN Neuro. 5:e00125. PubMed

RRID

AB_2565379 (BioLegend Cat. No. 837601) AB_2565380 (BioLegend Cat. No. 837602)

Antigen Details

Structure

GFAP is a 432 amino acid protein with a molecular mass of ~50 kD.

Distribution

Tissue distribution: GFAP is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, ependymal cells, and Bergmann glia cells (protoplasmic astrocyte). GFAP is expressed in cells lacking fibronectin.
Cellular distribution: Cytoskeleton and cytosol

Function

GFAP is a class-III intermediate filament and a structural constituent of the cytoskeleton. It is a cell-specific marker that is used to distinguish astrocytes from other glial cells during the development of the CNS.

Cell Type

Astrocytes

Biology Area

Cell Biology, Neuroscience, Neuroscience Cell Markers

Molecular Family

Intermediate Filaments

Antigen References