Anti-GFAP Antibody (Previously Covance catalog# SM-上海雅吉生物科技有限公司

规格	价格	库存
100µL	￥ 4340	1

Product Details

Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Ascites Fluid (contains 0.01M sodium azide).
Preparation: Ascites
Concentration: The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling: Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application: IHC-P - Quality tested
WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:1000 - 1:2000 is suggested.?For western blotting, the suggested use of this reagent is 1:1000 - 1:2000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Monoclonal antibody against glial fibrillary acidic protein (GFAP) derived from the Bigner-Eng clone MAb2E1.

SMI 24 provides for general visualization of GFAP in astrocytes and Bergman glia, it detects a more selected group of astrocytomas than mixtures of SMI 23, 24 and 25. The significance of this selectivity has not yet been explored. Possibilities are different characteristics of individual tumors or cooperativity of antibodies.

Multiple protein fragments ranging from 38 to 48 kD have been reported in human CNS lysates resulting from caspase- and calpain-mediated cleavage of GFAP.
RRID: AB_2565375 (BioLegend Cat. No. 837401)

Antigen Details

Structure

GFAP is a 432 amino acid protein with a molecular mass of approximately 50 kD.

Distribution

GFAP is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, ependymal cells, and Bergmann glia cells (unipolar protoplasmic astrocyte). GFAP is expressed in cells lacking fibronectin.

Function

GFAP is a class-III intermediate filament and a structural constituent of the cytoskeleton. It is a cell-specific marker that is used to distinguish astrocytes from other glial cells during the development of the CNS.

Cell Sources

Cytoskeleton and cytosol

Biology Area

Cell Biology, Neuroscience, Neuroscience Cell Markers

Molecular Family

Intermediate Filaments

Antigen References