Anti-GFAP (Cocktail) (Previously Covance catalog#
- 产品名称：
- Anti-GFAP (Cocktail) (Previously Covance catalog#
- 产品类别：
- 抗体
- 产品编号：
- 835301
- 产品应用：
- 835301
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Ascites Fluid (contains 0.01M sodium azide).
- Preparation
- Ascites
- Concentration
- The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
- Storage & Handling
- Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
- Application
-
IHC-P - Quality tested
ICC, WB - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:1000 – 1:2000 is suggested. For western blotting, a dilution range of 1:1000 – 1:2000 is suggested.? For immunocytochemistry, a dilution range of 1:1000 – 1:2000 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Multiple protein fragments ranging from 38 to 48 kD have been reported in human CNS lysates resulting from caspase- and calpain-mediated cleavage of GFAP.
- Application References
(PubMed link indicates BioLegend citation) -
- Kanno H, et al. 2014. J. Neurosci. 5:1838. (IHC) PubMed
- Vergo S, et al. 2011. Brain 134:571. (IHC)
- Jiang H, et al. 2012. Neurology 17:1767. (IHC, IP, WB) PubMed
- Traka M, et al. 2008. J. Neurosci. 45:11537. (IHC-P, WB) PubMed
- Bourque SL, et al. 2013. PLoS One 4:e61861. (IHC-F) PubMed
- Product Citations
-
- Abey A, et al. 2020. Brain Pathol. 31:144. PubMed
- Jiang H, et al. 2012. Neurology. 79:1767-1773. PubMed
- Lin N, et al. 2016. Mol Biol Cell. 27(25):3980-3990. PubMed
- Evonuk KS, et al. 2020. Sci Adv. 6:eaax5936. PubMed
- Traka M, et al. 2008. J Neurosci. 28:11537-11549. PubMed
- Bourque S, et al. 2013. PLoS One. 8:e61861. PubMed
- Care RA et al. 2019. Cell Rep. 27(7):2171-2183 . PubMed
- Zelikoff JT, et al. 2018. Toxicol Sci. 162:276. PubMed
- Huang S, et al. 2020. Cell Rep. 33:108394. PubMed
- Polis B, et al. 2018. Neurotherapeutics. 15:1036. PubMed
- Yu K, et al. 2016. Nat Mater. 10.1038/nmat4624. PubMed
- Kanno H, et al. 2014. J Neurosci. 34:1838-1855. PubMed
- Boscher E, et al. 2021. Front Neurosci. 14:591138. PubMed
- RRID
- AB_2565344 (BioLegend Cat. No. 835301)
- Structure
- GFAP is a 432 amino acid protein with a molecular mass of ~50 kD.
- Distribution
-
Tissue distribution: GFAP is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, ependymal cells, and Bergmann glia cells (protoplasmic astrocyte). GFAP is expressed in cells lacking fibronectin.
Cellular distribution: Cytoskeleton and cytosol
- Function
- GFAP is a class-III intermediate filament and a structural constituent of the cytoskeleton. It is a cell-specific marker that is used to distinguish astrocytes from other glial cells during the development of the CNS.
- Cell Type
- Astrocytes
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Antigen References
-
- van Bodegraven EJ, et al. 2019. Glia. 10.1002/glia.23594.
- Pekny M, et al. 2019. Neurosci Lett. 689:45.
- Hol EM, et al. 2017. Cold Spring Harb Perspect Biol. 9(12)
- Gene ID
- 2670 View all products for this Gene ID
- UniProt
- View information about GFAP on UniProt.org
