Purified anti-human CD3 Antibody
- 产品名称：
- Purified anti-human CD3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 344801
- 产品应用：
- 344801
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
ICC, IHC-F, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5,8, immunofluorescent staining6, and Western blotting3.
- Application References
(PubMed link indicates BioLegend citation) -
- Kan EA, et al. 1983. J. Immunol. 131:536.
- Wood GS, et al. 1985. Am. J. Pathol. 120:371.
- Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
- Haringman JJ, et al. 2005. Arthritis Res. Ther. 7:R862. (IHC)
- Carbone A, et al. 1999. Blood 93:2319. (IHC)
- Goval JJ, et al. 2006. J. Histochem. Cytochem. 54:75. (IF)
- Rutjens E, et al. 2007. J. Immunol. 178:1702.
- Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Product Citations
-
- Abdel-Gadir A, et al. 2019. Nat Med. 25:1164. PubMed
- Meckiff BJ, et al. 2019. J Immunol. 203:1276. PubMed
- Brinkmann CR, et al. 2018. mSphere. 3:e00616. PubMed
- Jacob F, et al. 2020. Cell. 180(1):188-204.e22.. PubMed
- Backes CS, et al. 2018. J Biol Chem. 293:16348. PubMed
- Jacob F, et al. 2020. Nat Protoc. 15:4000. PubMed
- Li Y, et al. 2019. Front Immunol. 0.460416667. PubMed
- Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed
- Murayama M, et al. 2015. Nat Commun. 6: 8483. PubMed
- Eriksson E, et al. 2017. Clin Cancer Res. 5.018055556. PubMed
- Xie CB, et al. 2020. J Clin Invest. 130:3437. PubMed
- RRID
- AB_2043994 (BioLegend Cat. No. 344801) AB_2043995 (BioLegend Cat. No. 344802)
- Structure
- Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ, (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
- Distribution
-
Mature T and NK T cells, during thymocyte differentiation
- Function
- Antigen recognition, signal transduction, T cell activation
- Ligand/Receptor
- Peptide antigen bound to MHC
- Cell Type
- NKT cells, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501.
- Gene ID
- 916 View all products for this Gene ID
- UniProt
- View information about CD3 on UniProt.org
