Alexa Fluor® 700 anti-human CD3 Antibody
- 产品名称：
- Alexa Fluor® 700 anti-human CD3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 344821
- 产品应用：
- 344821
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1736 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5,8, immunofluorescent staining6, and Western blotting3.
- Application References
(PubMed link indicates BioLegend citation) -
- Kan EA, et al. 1983. J. Immunol. 131:536.
- Wood GS, et al. 1985. Am. J. Pathol. 120:371.
- Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
- Haringman JJ, et al. 2005. Arthritis Res. Ther. 7:R862. (IHC)
- Carbone A, et al. 1999. Blood 93:2319. (IHC)
- Goval JJ, et al. 2006. J. Histochem. Cytochem. 54:75. (IF)
- Rutjens E, et al. 2007. J. Immunol. 178:1702.
- Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Product Citations
-
- Wang X, et al. 2019. Cell Res. 29:787. PubMed
- Montes de Oca M, et al. 2016. Cell Rep. 17:399-412. PubMed
- Zhao Y, et al. 2021. Front Immunol. 12:665442. PubMed
- Pachnio A, et al. 2016. PLoS Pathog. 12: 1005832. PubMed
- Fan P, et al. 2021. Front Oncol. 11:678758. PubMed
- van der Ploeg K, et al. 2022. Cell Rep Med. 3:100640. PubMed
- Li X, et al. 2019. Front Oncol. 1.115277778. PubMed
- Rice TF, et al. 2021. EBioMedicine. 72:103612. PubMed
- Yin W, et al. 2021. Thorac Cancer. 12:2680. PubMed
- You M, et al. 2021. Nat Cell Biol. 23:620. PubMed
- Hu EY, et al. 2020. JCI Insight. 5:00. PubMed
- Davis CW et al. 2019. Cell. 177(6):1566-1582 . PubMed
- Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
- Anderson AE, et al. 2022. NPJ Regen Med. 7:6. PubMed
- Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
- Collins DR, et al. 2021. Immunity. 54:2372. PubMed
- Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
- Gamradt S, et al. 2021. iScience. 24:103312. PubMed
- RRID
- AB_2563419 (BioLegend Cat. No. 344821) AB_2563420 (BioLegend Cat. No. 344822)
- Structure
- Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ, (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
- Distribution
-
Mature T and NK T cells, during thymocyte differentiation
- Function
- Antigen recognition, signal transduction, T cell activation
- Ligand/Receptor
- Peptide antigen bound to MHC
- Cell Type
- NKT cells, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501.
- Gene ID
- 916 View all products for this Gene ID
- UniProt
- View information about CD3 on UniProt.org
