Purified anti-human CD8 Antibody
- 产品名称：
- Purified anti-human CD8 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 344702
- 产品应用：
- 344702
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Chimpanzee, Pigtailed Macaque, Sooty Mangabey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10.?This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
- Campanelli R, et al. 2002. Intl. Immunol. 14:39.
- Evans RL, et al. 1981. Immunol. 78:544.
- Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
- Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
- Carbone A, et al. 1999. Blood 93:2319. (IHC-F)
- Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
- Nguyen LT, et al. 2019. Cancer Immunol Immunother. 68:773. PubMed
- Santos R, et al. 2017. Nat Commun. . 10.1038/s41467-017-01760-5. PubMed
- Yamaguchi A, et al. 2021. J Thorac Dis. 13:5430. PubMed
- Mann ER, et al. 2020. Sci Immunol. :5. PubMed
- Geng J, et al. 2018. Elife. 7:e36341. PubMed
- Oberg HH, et al. 2020. J Leukoc Biol. 107:1081. PubMed
- Jilg N, et al. 2020. J Infect Dis. 222:1837. PubMed
- Li H, et al. 2020. Mol Ther Methods Clin Dev. 1.436111111. PubMed
- Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
- Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed
- Gunawan M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16999-7. PubMed
- Park JB, et al. 2019. Mol Cells. 42:597. PubMed
- Cobb DA, et al. 2022. J Immunother Cancer. 10:. PubMed
- Cosgrove PR, et al. 2021. Pediatr Res. . PubMed
- Olin A, et al. 2018. Cell. 174:1277. PubMed
- Zhao J, et al. 2017. Sci Immunol. 2:eaan5400. PubMed
- Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
- Henrick BM, et al. 2021. Cell. . PubMed
- Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
- Bak S, et al. 2019. Front Immunol. 9:2953. PubMed
- Lakshmikanth T, et al. 2020. Cell Reports. 32(3):107923. PubMed
- Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
- Xie CB, et al. 2020. J Clin Invest. 130:3437. PubMed
- Ravell JC, et al. 2020. J Clin Invest. 130:507. PubMed
- Chiou SH, et al. 2021. Immunity. 54:586. PubMed
- RRID
- AB_1877104 (BioLegend Cat. No. 344702)
- Structure
- Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- NK cells, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8 on UniProt.org
