PerCP/Cyanine5.5 anti-human CD8 Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-human CD8 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 344709
- 产品应用：
- 344709
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2464 |
1 |
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Chimpanzee, Pigtailed Macaque, Sooty Mangabey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10.?This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
- Campanelli R, et al. 2002. Intl. Immunol. 14:39.
- Evans RL, et al. 1981. Immunol. 78:544.
- Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
- Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
- Carbone A, et al. 1999. Blood 93:2319. (IHC-F)
- Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Fang L, et al. 2013. Neuro Oncology. 15:1479. PubMed
- S?gaard O, et al. 2015. PLoS One. 11: 1005142. PubMed
- Wong SS, et al. 2021. Cell Reports Medicine. 2(4):100237. PubMed
- Koelsch K, et al. 2013. PLoS One. 8:50068. PubMed
- Khamaikawin W, et al. 2018. Mol Ther Methods Clin Dev. 9:23. PubMed
- Tang-Huau TL, et al. 2018. Nat Commun. 9:2570. PubMed
- Peng S et al. 2019. Cell Rep. 28(10):2728-2738 . PubMed
- Soldi R, et al. 2020. PLoS One. 15:e0235705. PubMed
- Parenti S, et al. 2021. NPJ Precis Oncol. 5:4. PubMed
- Mineo M, et al. 2020. Molecular Cell. 78(6):1207-1223.e8. PubMed
- Tatovic D, et al. 2015. J Immunol. 195: 386 - 392. PubMed
- Minagawa A, et al. 2018. Cell Stem Cell. 1.548611111. PubMed
- García-Ríos E, et al. 2022. Biomedicines. 10:. PubMed
- Basar R, et al. 2021. Cell Reports. 36(3):109432. PubMed
- You M, et al. 2021. Nat Cell Biol. 23:620. PubMed
- Wang X, et al. 2021. Adv Sci (Weinh). 8:e2101447. PubMed
- Basar R, et al. 2020. bioRxiv. . PubMed
- Warmuth S, et al. 2022. Oncoimmunology. 10:2004661. PubMed
- Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed
- Harper JL, et al. 2020. Nat Med. 519:26. PubMed
- Kim D, et al. 2020. Nat Med. 26:236. PubMed
- Arakawa A, et al. 2021. J Immunol. 207:2235. PubMed
- Feng Y, et al. 2022. Life (Basel). 12:. PubMed
- Shehata L, et al. 2019. Cell Rep. 28:3300. PubMed
- Lin G, et al. 2021. Mol Med Rep. 23:. PubMed
- Ng KW, et al. 2019. eLife. 0.333333333333333. PubMed
- Ringpis G, et al. 2012. PLoS One. 7:e53492. PubMed
- Rappazzo CG, et al. 2022. Immunity. 55:1710. PubMed
- Fels JM, et al. 2021. Cell. 184(13):3486-3501.e21. PubMed
- Terzioglu G,et al. 2017. Mech Ageing Dev. . 10.1016/j.mad.2017.09.003. PubMed
- Zhang J, et al. 2022. iScience. 25:105340. PubMed
- Wang Y, et al. 2021. Cell Reports. 36(6):109516. PubMed
- Sakharkar M, et al. 2021. Sci Immunol. :6. PubMed
- Shehata L, et al. 2019. Nat Commun. 10:1126. PubMed
- RRID
- AB_2044009 (BioLegend Cat. No. 344709) AB_2044010 (BioLegend Cat. No. 344710)
- Structure
- Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- NK cells, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8 on UniProt.org
