FITC anti-human CD8 Antibody
- 产品名称：
- FITC anti-human CD8 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 344703
- 产品应用：
- 344703
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1694 |
1 |
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Chimpanzee, Pigtailed Macaque, Sooty Mangabey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10.?This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
- Campanelli R, et al. 2002. Intl. Immunol. 14:39.
- Evans RL, et al. 1981. Immunol. 78:544.
- Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
- Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
- Carbone A, et al. 1999. Blood 93:2319. (IHC-F)
- Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
- Shao L, et al. 2021. Cell Death Discov. 7:145. PubMed
- Miao L, et al. 2020. Theranostics. 0.7625. PubMed
- Wang F, et al. 2018. Oncogenesis. 7:41. PubMed
- Mastelic-Gavillet B, et al. 2019. J Immunother Cancer. 7:257. PubMed
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- Zuazo M, et al. 2019. EMBO Mol Med. 11(7). PubMed
- Guo J, et al. 2022. Front Cell Dev Biol. 9:775599. PubMed
- Wu W, et al. 2020. Oncol Lett. 0.91875. PubMed
- Sun B, et al. 2019. Cancer Immunol Res. 1.550694444. PubMed
- Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
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- Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
- Su X, et al. 2022. J Transl Med. 20:378. PubMed
- Lou Q, et al. 2019. J Immunother Cancer. 7:210. PubMed
- Gannon PO, et al. 2020. Cytotherapy. 22(12):780-791. PubMed
- Dai Z, et al. 2022. Signal Transduct Target Ther. 7:85. PubMed
- Xu C, et al. 2020. Methods Mol Biol. 2108:159. PubMed
- Martínez-Fábregas J, et al. 2020. Cell Rep. 33:108545. PubMed
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- Prota G, et al. 2020. Cancer Immunol Res. 8:685. PubMed
- Weiss R, et al. 2021. Cells. 10:. PubMed
- FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed
- Sam J, et al. 2020. Front Oncol. 10:575737. PubMed
- Barresi V, et al. 2020. J Clin Med. 9:00. PubMed
- Lu C, et al. 2020. Cancer Cell. 39(1):96-108.e6. PubMed
- Méndez-Lagares G, et al. 2021. J Clin Invest. 131:e148542. PubMed
- Gullà A, et al. 2021. Cancer Discov. 2:468. PubMed
- Loo Yau H, et al. 2021. STAR Protocols. 2(2):100549. PubMed
- Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
- Hu Q, et al. 2021. Nat Biomed Eng. 5:1038. PubMed
- Vanoni G, et al. 2021. eLife. 10:00. PubMed
- Goc J, et al. 2021. Cell. :. PubMed
- Li B, et al. 2019. Oncogenesis. 8:17. PubMed
- RRID
- AB_1877179 (BioLegend Cat. No. 344703) AB_1877178 (BioLegend Cat. No. 344704)
- Structure
- Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- NK cells, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8 on UniProt.org
