APC anti-human CD8 Antibody
- 产品名称：
- APC anti-human CD8 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 344721
- 产品应用：
- 344721
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1848 |
1 |
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Chimpanzee, Pigtailed Macaque, Sooty Mangabey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10.?This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
- Campanelli R, et al. 2002. Intl. Immunol. 14:39.
- Evans RL, et al. 1981. Immunol. 78:544.
- Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
- Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
- Carbone A, et al. 1999. Blood 93:2319. (IHC-F)
- Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Wu Y, et al. 2021. Cancer Gene Ther. Online ahead of print. PubMed
- Wang F, et al. 2018. Oncogenesis. 7:41. PubMed
- Tang L, et al. 2021. Oncol Lett. 22:605. PubMed
- Wahl S, et al. 2016. Nature. 541:81-86. PubMed
- Lugo-Reyes SO, et al. 2021. J Clin Immunol. 41:1291. PubMed
- Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
- Zhao L, et al. 2020. Mol Ther Oncolytics. 16:262. PubMed
- Kasper M, et al. 2021. Elife. 10:. PubMed
- Massafra V, et al. 2021. J Immunol. 207:493. PubMed
- Reif T, et al. 2021. Cell Reports Medicine. 2(6):100317. PubMed
- Kang L, et al. 2020. Exp Hematol Oncol. 9:11. PubMed
- Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
- Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
- Kumar A, et al. 2020. Bio Protoc. 10:e3675. PubMed
- Qiu C, et al. 2022. Front Immunol. 12:764949. PubMed
- Zou F, et al. 2021. Mol Ther. 29:1794. PubMed
- Leitner J, et al. 2015. J Immunol. 195: 477 - 487. PubMed
- Jing L, et al. 2020. Cancer Immunol Res. 8:648. PubMed
- Thiel BA, et al. 2021. PLoS One. 16:e0249477. PubMed
- Su S et al. 2018. Cell. 175(2):442-457 . PubMed
- Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
- Wang J, et al. 2019. Oncol Lett. 4.948611111. PubMed
- Bear AS, et al. 2021. Nat Commun. 12:4365. PubMed
- Mudd PA, et al. 2022. Cell. 185:603. PubMed
- Bonte S, et al. 2021. Oncoimmunology. 10:1954800. PubMed
- Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed
- Horn LA, et al. 2017. Oncotarget. 8:57964. PubMed
- Paul S, et al. 2021. Sci Transl Med. 13:. PubMed
- Grant RA, et al. 2021. Nature. 590(7847):635-641. PubMed
- Chheda ZS, et al. 2018. J Exp Med. 9:19490. PubMed
- Egedal JH, et al. 2021. Mucosal Immunol. 14:1203. PubMed
- Roudko V, et al. 2020. Cell. 183(6):1634-1649.e17. PubMed
- Su Y, et al. 2020. Cell. 1479:183. PubMed
- Zheng Z, et al. 2021. Biomed Res Int. 2021:5535578. PubMed
- Nguyen NT, et al. 2021. Nat Nanotechnol. 16:1424. PubMed
- Loo Yau H, et al. 2021. STAR Protocols. 2(2):100549. PubMed
- Clayton KL, et al. 2021. Cell Host Microbe. 29(3):435-447.e9. PubMed
- Tao L, et al. 2020. Cancers (Basel). 12:00. PubMed
- Oliveira A, et al. 2013. Immunology. 140:47. PubMed
- Fierle JK, et al. 2021. Cell Reports Medicine. 2(8):100362. PubMed
- Hoseini SS, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(5):. PubMed
- Walker‐Sperling VE et al. 2017. EBioMedicine. 16:141-149 . PubMed
- Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed
- Javed A, et al. 2015. PLoS One. 10: 0142086. PubMed
- Forsberg EMV, et al. 2019. Cancer Res. 79:899. PubMed
- Li B, et al. 2019. Oncogenesis. 8:17. PubMed
- Feng Y, et al. 2022. EClinicalMedicine. 43:101226. PubMed
- Bhargavan B, et al. 2021. Mol Neurodegener. 16:78. PubMed
- RRID
- AB_2075390 (BioLegend Cat. No. 344721) AB_2075388 (BioLegend Cat. No. 344722)
- Structure
- Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- NK cells, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8 on UniProt.org
