Alexa Fluor® 700 anti-human CD8 Antibody
- 产品名称：
- Alexa Fluor® 700 anti-human CD8 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 344723
- 产品应用：
- 344723
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1568 |
1 |
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Chimpanzee, Pigtailed Macaque, Sooty Mangabey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10.?This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
- Campanelli R, et al. 2002. Intl. Immunol. 14:39.
- Evans RL, et al. 1981. Immunol. 78:544.
- Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
- Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
- Carbone A, et al. 1999. Blood 93:2319. (IHC-F)
- Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Charpentier JC, et al. 2020. Nat Commun. 11:180. PubMed
- Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
- Crespo ?C, et al. 2020. Cell. 182(5):1125-1139. PubMed
- Yarzabek B et al. 2018. eLife. 7 pii: e34961. PubMed
- Neidleman J, et al. 2020. Cell Rep Med. 100081:1. PubMed
- Pellett Madan R, et al. 2020. Clin Transplant. 34:e14021. PubMed
- Ma X, et al. 2021. Cell Metabolism. 33(5):1001-1012.e5. PubMed
- Ott PA, et al. 2020. Cell. 183(2):347-362.e24. PubMed
- Serra EG, et al. 2020. Nat Commun. 11:995. PubMed
- Ackerley CG, et al. 2022. Front Immunol. 13:972170. PubMed
- Williamson LM, et al. 2021. NPJ Precis Oncol. 5:103. PubMed
- Vanoni G, et al. 2022. Bio Protoc. 218:. PubMed
- Cohen CA, et al. 2021. Nat Commun. 12:4678. PubMed
- Coufal S, et al. 2019. Cells. 8. PubMed
- Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed
- Bradley D, et al. 2022. Nat Commun. 13:5606. PubMed
- FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed
- Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
- Zhong W, et al. 2022. Front Immunol. 13:1001255. PubMed
- RRID
- AB_2562789 (BioLegend Cat. No. 344723) AB_2562790 (BioLegend Cat. No. 344724)
- Structure
- Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- NK cells, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8 on UniProt.org
