Alexa Fluor® 488 anti-human CD8 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100tests	￥ 4522	1

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Chimpanzee, Pigtailed Macaque, Sooty Mangabey

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested
SB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

*Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Blue Laser (488 nm)

Application Notes

Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics⁸, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)^9,10.?This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References (PubMed link indicates BioLegend citation)

Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
Campanelli R, et al. 2002. Intl. Immunol. 14:39.
Evans RL, et al. 1981. Immunol. 78:544.
Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
Carbone A, et al. 1999. Blood 93:2319. (IHC-F)
Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)
Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed

Product Citations

Chan S, et al. 2022. Nat Cancer. . PubMed
Peng S et al. 2019. Cell Rep. 28(10):2728-2738 . PubMed
Li N, et al. 2021. Cell Rep Med. 2:100297. PubMed
Chapelin F, et al. 2017. Sci Rep.. 10.1038/s41598-017-17669-4. PubMed

RRID

AB_10549301 (BioLegend Cat. No. 344716)

Antigen Details

Structure: Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
Distribution: Majority of thymocytes, T cell subset, NK cells
Function: MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor: MHC Class I molecules
Cell Type: NK cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
Gene ID: 925 View all products for this Gene ID
UniProt: View information about CD8 on UniProt.org

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