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PE/Dazzle™ 594 anti-mouse H-2K
产品名称:
PE/Dazzle™ 594 anti-mouse H-2K
产品类别:
抗体
产品编号:
116633
产品应用:
116633
[价格]
规格 价格 库存
25µg ¥ 1652 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BALB/c Mouse cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle? 594 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle? 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The SF1-1.1 antibody is weakly cross-reactive with H-2k but does not cross-react with other haplotypes (b, j, p, q, s, v). Clone SF1-1.1 recognizes the a3 domain of Kd.

Additional reported applications (for the relevant formats) include: immunoprecipitation1,4 and Western blotting2.

Application References

(PubMed link indicates BioLegend citation)
  1. Noun G, et al. 1996. J. Immunol. 157:2455. (IP)
  2. Abastado JP, et al. 1993. J. Immunol. 151:3569. (WB)
  3. Bashuda H, et al. 1997. Transplantation 63:113.
  4. Sester M, et al. 2000. J. Biol. Chem. 34:113. (IP)
  5. Ma XT, et al. 2006. Cancer Res. 66:1169. (FC)
  6. Norian LA and Allen PM. 2004. J. Immunol. 173:835. (FC)
  7. Norian L, et al. 2004. J. Immunol.. 173:835. PubMed
  8. Delon J, et al. 1998. Immunity 9:467.
RRID
AB_2832407 (BioLegend Cat. No. 116633) AB_2832408 (BioLegend Cat. No. 116634)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Kd haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References
  1. Watts C. 1997. Annu. Rev. Immunol. 15:821.
  2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.
  3. York I, et al. 1996. Annu. Rev. Immunol. 14:369.
Gene ID
14972 View all products for this Gene ID
UniProt
View information about H-2Kd on UniProt.org

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