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首页 > 抗体蛋白 > 抗体
Pacific Blue™ anti-mouse H-2K
产品名称：
Pacific Blue™ anti-mouse H-2K
产品类别：
抗体
产品编号：
116615
产品应用：
116615
[价格]
规格 价格 库存
25µg ￥ 1568 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BALB/c Mouse cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

The SF1-1.1 antibody is weakly cross-reactive with H-2k but does not cross-react with other haplotypes (b, j, p, q, s, v). Clone SF1-1.1 recognizes the a3 domain of Kd.

Additional reported applications (for the relevant formats) include: immunoprecipitation1,4 and Western blotting2.

Application References

(PubMed link indicates BioLegend citation)
  1. Noun G, et al. 1996. J. Immunol. 157:2455. (IP)
  2. Abastado JP, et al. 1993. J. Immunol. 151:3569. (WB)
  3. Bashuda H, et al. 1997. Transplantation 63:113.
  4. Sester M, et al. 2000. J. Biol. Chem. 34:113. (IP)
  5. Ma XT, et al. 2006. Cancer Res. 66:1169. (FC)
  6. Norian LA and Allen PM. 2004. J. Immunol. 173:835. (FC)
  7. Norian L, et al. 2004. J. Immunol.. 173:835. PubMed
  8. Delon J, et al. 1998. Immunity 9:467.
Product Citations
  1. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  2. Skowron K, et al. 2016. Sci Rep. 6:35854. PubMed
  3. Chen H, et al. 2005. J Immunol. 175:591. PubMed
  4. Sperber HS, et al. 2020. FASEB J. 14615:34. PubMed
  5. Sim?es BM, et al. 2020. Oncogene. 39:4896. PubMed
  6. Allen L 2004. J Immunol. 173:835. PubMed
RRID
AB_1937243 (BioLegend Cat. No. 116615) AB_1937242 (BioLegend Cat. No. 116616)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Kd haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References
  1. Watts C. 1997. Annu. Rev. Immunol. 15:821.
  2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.
  3. York I, et al. 1996. Annu. Rev. Immunol. 14:369.
Gene ID
14972 View all products for this Gene ID
UniProt
View information about H-2Kd on UniProt.org

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