Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

The SF1-1.1 antibody is weakly cross-reactive with H-2^k but does not cross-react with other haplotypes (b, j, p, q, s, v). Clone SF1-1.1 recognizes the a3 domain of K^d.

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,4 and Western blotting².

1. Noun G, et al. 1996. J. Immunol. 157:2455. (IP)
2. Abastado JP, et al. 1993. J. Immunol. 151:3569. (WB)
3. Bashuda H, et al. 1997. Transplantation 63:113.
4. Sester M, et al. 2000. J. Biol. Chem. 34:113. (IP)
5. Ma XT, et al. 2006. Cancer Res. 66:1169. (FC)
6. Norian LA and Allen PM. 2004. J. Immunol. 173:835. (FC)
7. Norian L, et al. 2004. J. Immunol.. 173:835. PubMed
8. Delon J, et al. 1998. Immunity 9:467.

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18. Huanga J, et al. 2013. J Immunol Methods. 387:254. PubMed

Nucleated cells of the H-2K^d haplotype

1. Watts C. 1997. Annu. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.
3. York I, et al. 1996. Annu. Rev. Immunol. 14:369.