Brilliant Violet 421™ anti-mouse H-2K-上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 4004	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

BALB/c Mouse cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

The SF1-1.1 antibody is weakly cross-reactive with H-2^k but does not cross-react with other haplotypes (b, j, p, q, s, v). Clone SF1-1.1 recognizes the a3 domain of K^d.

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,4 and Western blotting².

Application References (PubMed link indicates BioLegend citation)

Noun G, et al. 1996. J. Immunol. 157:2455. (IP)
Abastado JP, et al. 1993. J. Immunol. 151:3569. (WB)
Bashuda H, et al. 1997. Transplantation 63:113.
Sester M, et al. 2000. J. Biol. Chem. 34:113. (IP)
Ma XT, et al. 2006. Cancer Res. 66:1169. (FC)
Norian LA and Allen PM. 2004. J. Immunol. 173:835. (FC)
Norian L, et al. 2004. J. Immunol.. 173:835. PubMed
Delon J, et al. 1998. Immunity 9:467.

RRID

AB_2565656 (BioLegend Cat. No. 116623)

Antigen Details

Structure

MHC class I

Distribution

Nucleated cells of the H-2K^d haplotype

Function

Antigen presentation

Ligand/Receptor

CD3/TCR, CD8

Biology Area

Immunology, Innate Immunity

Molecular Family

MHC Antigens

Antigen References

Watts C. 1997. Annu. Rev. Immunol. 15:821.
Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.
York I, et al. 1996. Annu. Rev. Immunol. 14:369.

Gene ID

14972 View all products for this Gene ID

UniProt

View information about H-2Kd on UniProt.org

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