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Biotin anti-mouse H-2K
产品名称:
Biotin anti-mouse H-2K
产品类别:
抗体
产品编号:
116603
产品应用:
116603
[价格]
规格 价格 库存
50µg ¥ 1162 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BALB/c Mouse cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.?For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The SF1-1.1 antibody is weakly cross-reactive with H-2k but does not cross-react with other haplotypes (b, j, p, q, s, v). Clone SF1-1.1 recognizes the a3 domain of Kd.

Additional reported applications (for the relevant formats) include: immunoprecipitation1,4 and Western blotting2.

Application References

(PubMed link indicates BioLegend citation)
  1. Noun G, et al. 1996. J. Immunol. 157:2455. (IP)
  2. Abastado JP, et al. 1993. J. Immunol. 151:3569. (WB)
  3. Bashuda H, et al. 1997. Transplantation 63:113.
  4. Sester M, et al. 2000. J. Biol. Chem. 34:113. (IP)
  5. Ma XT, et al. 2006. Cancer Res. 66:1169. (FC)
  6. Norian LA and Allen PM. 2004. J. Immunol. 173:835. (FC)
  7. Norian L, et al. 2004. J. Immunol.. 173:835. PubMed
  8. Delon J, et al. 1998. Immunity 9:467.
Product Citations
  1. Yen W, et al. 2012. Clin Cancer Res. 18:5374. PubMed
  2. Zabala M, et al. 2020. Cell Stem Cell. 27(2):284-299.e8. PubMed
  3. Culina S, et al. 2014. PLoS One. 9:88365. PubMed
  4. Gross ETE, et al. 2018. Oncoimmunology. 8:e1404212. PubMed
  5. Ishay–Ronen D, et al. 2019. Cancer Cell. 35:17. PubMed
  6. Uno N, et al. 2015. Mol Ther Nucleic Acids. 4, e272. PubMed
  7. Agudo J et al. 2018. Immunity. 48(2):271-285 . PubMed
  8. Allen L 2004. J Immunol. 173:835. PubMed
RRID
AB_313738 (BioLegend Cat. No. 116603) AB_313739 (BioLegend Cat. No. 116604)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Kd haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References
  1. Watts C. 1997. Annu. Rev. Immunol. 15:821.
  2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.
  3. York I, et al. 1996. Annu. Rev. Immunol. 14:369.
Gene ID
14972 View all products for this Gene ID
UniProt
View information about H-2Kd on UniProt.org

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