Biotin anti-human CD172a/b (SIRPα/β) An-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2954	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

NIH-3T3/hu-SIRPα cell line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone SE5A5 recognizes a common epitope on SIRPa (90 kD) and SIRP? (50 kD)³. A high degree of homology has been found between SIRP family isoforms alpha and beta at the level of extracellular domains. Consequently, many anti SIRP antibody clones, such as SE5A5, have been reported to cross react with several SIRP isoforms^1,4,5. It reacts with CD172a and has weak cross-reaction with CD172b. This antibody is able to block the binding of SIRPa (SIRPa1 and SIRPa2) to CD47^1,6.

Application References (PubMed link indicates BioLegend citation)

Seiffert M, et al. 1999. Blood 94:3633.
Dubois NC, et al. 2011. Nat. Biotechnol. 29:1011.
Barros MM, et al. 2009. Transfusion 49:154.
Liu Y, et al. 2005. J. Biol. Chem. 280:36132.
Barclay AN. 2009. Curr. Opin. Immunol. 21:47.
Florian S, et al. 2005. J. Leukoc. Biol. 77:984.

Product Citations

Mittag D, et al. 2011. J Immunol. 186:6207. PubMed

RRID

AB_830703 (BioLegend Cat. No. 323804)

Antigen Details

Structure: 75-110 kD glycoprotein , signal-regulatorey-protein family, Ig superfamily
Distribution: Monocytes/macrophages, granulocytes, dendritic cells, neurons, cardiomyocytes
Function: Negative regulation of leukocyte adhesion and transmigration, macrophage fusion, phagocytosis
Ligand/Receptor: CD47
Cell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes, Neurons
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Seiffert M, et al. 1999. Blood 94:3633.
2. Seiffert M, et al. 2001. Blood 97:2741.
3. Timms JF, et al. 1998. Mol. Cell Biol. 18:3838.
4. Barclay AN and Brown MH. 2006. Nat. Rev. Immnuol. 6:457.
Gene ID: 140885 View all products for this Gene ID
UniProt: View information about CD172alpha/beta on UniProt.org

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