Alexa Fluor® 700 anti-human CD172a/b (SIRP&alp-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1904	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

NIH-3T3/hu-SIRPα cell line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Clone SE5A5 recognizes a common epitope on SIRPa (90 kD) and SIRP? (50 kD)³. A high degree of homology has been found between SIRP family isoforms alpha and beta at the level of extracellular domains. Consequently, many anti SIRP antibody clones, such as SE5A5, have been reported to cross react with several SIRP isoforms^1,4,5. It reacts with CD172a and has weak cross-reaction with CD172b. This antibody is able to block the binding of SIRPa (SIRPa1 and SIRPa2) to CD47^1,6.

Application References (PubMed link indicates BioLegend citation)

Seiffert M, et al. 1999. Blood 94:3633.
Dubois NC, et al. 2011. Nat. Biotechnol. 29:1011.
Barros MM, et al. 2009. Transfusion 49:154.
Liu Y, et al. 2005. J. Biol. Chem. 280:36132.
Barclay AN. 2009. Curr. Opin. Immunol. 21:47.
Florian S, et al. 2005. J. Leukoc. Biol. 77:984.

Product Citations

Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed

RRID

AB_2687274 (BioLegend Cat. No. 323815) AB_2687275 (BioLegend Cat. No. 323816)

Antigen Details

Structure: 75-110 kD glycoprotein , signal-regulatorey-protein family, Ig superfamily
Distribution: Monocytes/macrophages, granulocytes, dendritic cells, neurons, cardiomyocytes
Function: Negative regulation of leukocyte adhesion and transmigration, macrophage fusion, phagocytosis
Ligand/Receptor: CD47
Cell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes, Neurons
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Seiffert M, et al. 1999. Blood 94:3633.
2. Seiffert M, et al. 2001. Blood 97:2741.
3. Timms JF, et al. 1998. Mol. Cell Biol. 18:3838.
4. Barclay AN and Brown MH. 2006. Nat. Rev. Immnuol. 6:457.
Gene ID: 140885 View all products for this Gene ID
UniProt: View information about CD172alpha/beta on UniProt.org

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