PE anti-mouse CD127 (IL-7Rα) Antibody
- 产品名称：
- PE anti-mouse CD127 (IL-7Rα) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 121111
- 产品应用：
- 121111
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse pre-B cell line 1A9
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining.
- Application References
(PubMed link indicates BioLegend citation) -
- Yamashita Y, et al. 1999. J. Immunol. 162:5940.
- Kouro T, et al. 2002. Blood 100:3672.
- Maeda K, et al. 2005. Blood 106:879.
- Diao J, et al. 2004. J. Immunol. 173:1826.
- Product Citations
-
- Schmidt N, et al. 2008. Proc Natl Acad Sci U S A. 105:14017. PubMed
- Sch?nberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
- MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
- Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
- Paubelle E, et al. 2020. Cell Reports. 30(3):739-754.e4.. PubMed
- del Rio ML, et al. 2021. Transl Res. Online ahead of print.. PubMed
- Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
- Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
- Cuburu N, et al. 2019. J Immunol. 202:1250. PubMed
- McAlpine CS, et al. 2021. Nature. 595:701. PubMed
- Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
- Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
- Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
- Ding P, et al. 2022. Bone Res. 10:42. PubMed
- Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
- RRID
- AB_493510 (BioLegend Cat. No. 121111) AB_493509 (BioLegend Cat. No. 121112)
- Structure
- Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
- Distribution
-
Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
- Function
- T cell and immature B cell proliferation and development
- Ligand/Receptor
- IL-7
- Cell Type
- B cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Sudo T, et al. 1993. Proc. Natl. Acad. Sci. USA. 90:9125.
2. He YW and Malek TR. 1998. Crit Rev. Immunol. 18:503.
3. Huster K M,et al. 2004. Proc. Natl. Acad. Sci. USA. 101:5610.
4. Pillai M, et al. 2004. Leuk Lymphoma. 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
- Gene ID
- 16197 View all products for this Gene ID
- UniProt
- View information about CD127 on UniProt.org
