Purified anti-mouse CD284 (TLR4) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1078	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

LPS-stimulated TLR4/MD-2/CD14 cotransfected NKR cells.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.¹ Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.¹ Unlike MTS510, the binding of SA15-21 is not interrupted by LPS stimulation¹. Clone SA15-21 is capable of recognizing TLR4 individually and when it is complexed with MD-2.

Additional reported applications (for the relevant formats) include: immunoprecipitation¹ and inhibition of LPS-induced hepatocyte apoptosis². The LEAF? or Ultra-LEAF? purified antibody is recommended for functional assays (contact our custom solutions team).

Application References (PubMed link indicates BioLegend citation)

Akashi S, et al. 2003. J. Exp. Med. 198:1035. (IP)
Wakabayashi Y, et al. 2006. J. Immunol. 177:1772. (Block)
Visintin A, et al. 2006. J. Leukoc. Biol. 80:1584. (FC)
Shibata T, et al. 2011. Int. Immunol. 23:503. (FC)

Product Citations

Yang C, et al. 2019. MBio. 10:e00595-19. PubMed
Rosa JM, et al. 2021. British Journal of Pharmacology. . PubMed
Matsuba S, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01538. PubMed
Lim TJF, et al. 2020. J Exp Med. 217:00:00. PubMed

RRID

AB_2561871 (BioLegend Cat. No. 145401) AB_2561872 (BioLegend Cat. No. 145402)

Antigen Details

Structure: Type I transmembrane protein complex
Distribution: Macrophages, B cells and T cells
Interaction: Activates the innate immune system against Gram-negative pathogens by recognition of LPS
Ligand/Receptor: Bacterial LPS
Cell Type: Macrophages, B cells, T cells, Dendritic cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Toll Like Receptors
Antigen References: 1. Brennan TV, et al. 2012. Blood 120:2899.
2. Wantia N, et al. 2011. PLoS One 6:e26101.
3. Loser K, et al. 2010. Nat. Med. 16:713.
4. Kang S, et al. 2009. Blood 113:2605.
Gene ID: 21898 View all products for this Gene ID
UniProt: View information about CD284 on UniProt.org

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