PE/Cyanine7 anti-mouse CD284 (TLR4) Antibody
- 产品名称：
- PE/Cyanine7 anti-mouse CD284 (TLR4) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 145407
- 产品应用：
- 145407
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- LPS-stimulated TLR4/MD-2/CD14 cotransfected NKR cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.1 Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.1 Unlike MTS510, the binding of SA15-21 is not interrupted by LPS stimulation1. Clone SA15-21 is capable of recognizing TLR4 individually and when it is complexed with MD-2.
Additional reported applications (for the relevant formats) include: immunoprecipitation1 and inhibition of LPS-induced hepatocyte apoptosis2. The LEAF? or Ultra-LEAF? purified antibody is recommended for functional assays (contact our custom solutions team).
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Akashi S, et al. 2003. J. Exp. Med. 198:1035. (IP)
- Wakabayashi Y, et al. 2006. J. Immunol. 177:1772. (Block)
- Visintin A, et al. 2006. J. Leukoc. Biol. 80:1584. (FC)
- Shibata T, et al. 2011. Int. Immunol. 23:503. (FC)
- Product Citations
-
- Trittel S, et al. 2019. Sci Rep. 9:16362. PubMed
- Celorrio M, et al. 2022. Acta Neuropathol Commun. 10:10. PubMed
- Kothari V, et al. 2021. Arterioscler Thromb Vasc Biol. 41:1854. PubMed
- Fotakis P, et al. 2019. Arterioscler Thromb Vasc Biol. 39:e253. PubMed
- RRID
- AB_2562536 (BioLegend Cat. No. 145407) AB_2562537 (BioLegend Cat. No. 145408)
- Structure
- Type I transmembrane protein complex
- Distribution
- Macrophages, B cells and T cells
- Interaction
- Activates the innate immune system against Gram-negative pathogens by recognition of LPS
- Ligand/Receptor
- Bacterial LPS
- Cell Type
- Macrophages, B cells, T cells, Dendritic cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Toll Like Receptors
- Antigen References
-
1. Brennan TV, et al. 2012. Blood 120:2899.
2. Wantia N, et al. 2011. PLoS One 6:e26101.
3. Loser K, et al. 2010. Nat. Med. 16:713.
4. Kang S, et al. 2009. Blood 113:2605.
- Gene ID
- 21898 View all products for this Gene ID
- UniProt
- View information about CD284 on UniProt.org
