Purified anti-human CD88 (C5aR) Antibody
- 产品名称：
- Purified anti-human CD88 (C5aR) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 344302
- 产品应用：
- 344302
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant peptide huC5aR N-terminal -NT (Asp15-Asp27)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-P - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone S5/1 blocks the binding of C5a to CD88.
- Application References
(PubMed link indicates BioLegend citation) -
- Kiener HP, et al. 1998. Arthritis Rheum. 41:233.
- Elsner J, et al. 1994. Blood 83:3324.
- Oppermann M, et al. 1993. J. Immunol. 151:3785.
- Product Citations
-
- Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
- Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
- Brandolini L, et al. 2022. Cell Death Dis. 13:500. PubMed
- Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
- Colley CS, et al. 2018. MAbs. 10:104. PubMed
- Lee EKS, et al. 2018. Cell Host Microbe. 23:121. PubMed
- Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed
- Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
- RRID
- AB_2259318 (BioLegend Cat. No. 344302)
- Structure
- CD88 is a single chain protein with seven membrane-spanning regions and a MW of 43 kD.
- Distribution
-
CD88 is expressed by monocytes, neutrophils and eosinophils. There has been reports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells, cardiomyocytes and vascular endothelial cells.
- Function
- After C5a binding, the CD88 signal results in chemotaxis, granule enzyme release and superoxide anion production.
- Interaction
- CD88 is coupled to heterotrimeric G proteins such as Gi. The signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules.
- Cell Type
- Eosinophils, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Schraufstatter IU, et al. 2009. J. Immunol. 182:3827.
2. Ippel JH, et al. 2009. J. Biol Chem. 284:12363.
3. Griffin RS, et al. 2007. J. Neurosci. 27:8699.
4. Lee H, et al. 2006. Nat. Biotechnol. 24:1279.
5. Buck E and Wells JA. et al. 2005. P. Natl. Acad. Sci. USA 102:2719.
- Gene ID
- 728 View all products for this Gene ID
- UniProt
- View information about CD88 on UniProt.org
