PE/Dazzle™ 594 anti-human CD88 (C5aR) Antibo
- 产品名称：
- PE/Dazzle™ 594 anti-human CD88 (C5aR) Antibo
- 产品类别：
- 抗体
- 产品编号：
- 344318
- 产品应用：
- 344318
[价格]
| 规格 |
价格 |
库存 |
| 100tests |
￥ 5180 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant peptide huC5aR N-terminal -NT (Asp15-Asp27)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle? 594 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* PE/Dazzle? 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Clone S5/1 blocks the binding of C5a to CD88.
- Application References
(PubMed link indicates BioLegend citation) -
- Kiener HP, et al. 1998. Arthritis Rheum. 41:233.
- Elsner J, et al. 1994. Blood 83:3324.
- Oppermann M, et al. 1993. J. Immunol. 151:3785.
- Product Citations
-
- Liechti T, et al. 2022. Nat Commun. 13:7255. PubMed
- RRID
- AB_2750446 (BioLegend Cat. No. 344317) AB_2750447 (BioLegend Cat. No. 344318)
- Structure
- CD88 is a single chain protein with seven membrane-spanning regions and a MW of 43 kD.
- Distribution
-
CD88 is expressed by monocytes, neutrophils and eosinophils. There has been reports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells, cardiomyocytes and vascular endothelial cells.
- Function
- After C5a binding, the CD88 signal results in chemotaxis, granule enzyme release and superoxide anion production.
- Interaction
- CD88 is coupled to heterotrimeric G proteins such as Gi. The signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules.
- Cell Type
- Eosinophils, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Schraufstatter IU, et al. 2009. J. Immunol. 182:3827.
2. Ippel JH, et al. 2009. J. Biol Chem. 284:12363.
3. Griffin RS, et al. 2007. J. Neurosci. 27:8699.
4. Lee H, et al. 2006. Nat. Biotechnol. 24:1279.
5. Buck E and Wells JA. et al. 2005. P. Natl. Acad. Sci. USA 102:2719.
- Gene ID
- 728 View all products for this Gene ID
- UniProt
- View information about CD88 on UniProt.org
