Alexa Fluor® 647 anti-human CD44 variant 9 (CD-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2058	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Human CD44v8-10 transfected cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For immunohistochemistry, a concentration range of 5.0?- 10??g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections³.

Application References (PubMed link indicates BioLegend citation)

Kakehashi, A., et al. 2016. Cancer Sci. 107: 609. (IHC-P)
Aso T, et al. 2015. PLoS One. 10: e0116596. (IHC-P)
Yoshikawa M, et al. 2013. Cancer Res. 73: 1855. (IHC-F)
Ishimoto T, et al. 2011. Cancer Cell. 387-400. (IHC-P)

RRID

AB_2814346 (BioLegend Cat. No. 394407) AB_2814346 (BioLegend Cat. No. 394408)

Antigen Details

Distribution

Cancer stem cells, epithelial-type carcinomas, subset of monocytes, subset of lymphocytes.

Function

Role in tumor initiation and maintenance and metastasis of cancer cells, Interacts with the glutamate-cystine transporter xCT, increasing reduced glutathione and contributing to the ROS resistance of cancer cells.

Cell Type

Epithelial cells, Lymphocytes, Monocytes, Neutrophils

Biology Area

Cancer Biomarkers, Cell Biology, Immunology

Molecular Family

Adhesion Molecules, CD Molecules

Antigen References