PE anti-human CD8a Antibody
- 产品名称：
- PE anti-human CD8a Antibody
- 产品类别：
- 抗体
- 产品编号：
- 301007
- 产品应用：
- 301007
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Chimpanzee, Baboon, Pigtailed Macaque, Sooty Mangabey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- ?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). - Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- ?g sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections3 and costimulation of T cell responses4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
- Magidovich E, et al. 2007. P. Natl. Acad. Sci. USA 104:13022.
- Thakarl D, et al. 2008.J. immunol. 180:7431. PubMed
- Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Product Citations
-
- Penkava F, et al. 2020. Nat Commun. 3.76875. PubMed
- Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
- Wang T, et al. 2022. Front Immunol. 13:954121. PubMed
- Zong D, et al. 2021. BMC Biol. 19:79. PubMed
- Ding L, et al. 2021. Cancers (Basel). 13:. PubMed
- Wu SY, et al. 2021. J Immunother Cancer. 9: . PubMed
- Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
- Lo Presti V, et al. 2021. Mol Ther Methods Clin Dev. 21:357. PubMed
- Schupp J, et al. 2021. Int J Mol Sci. 22:00. PubMed
- Theobald SJ, et al. 2022. EMBO Mol Med. 14:e15888. PubMed
- VanSaun M, et al. 2013. PLoS One. 8:73054. PubMed
- Mozhgani SH, et al. 2019. Retrovirology. 16:46. PubMed
- Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
- Mohammadpour H, et al. 2021. Cell Rep. 37:109883. PubMed
- Calzoni E, et al. 2019. J Allergy Clin Immunol. 143:2317. PubMed
- Liu X, et al. 2020. Ther Adv Med Oncol. 12:1758835920910347. PubMed
- Reyes M, et al. 2021. Sci Transl Med. 13:. PubMed
- Ajith A, et al. 2019. FASEB J. 33:5220. PubMed
- Hebbandi Nanjundappa R, et al. 2017. Cell. 171:655. PubMed
- RRID
- AB_314125 (BioLegend Cat. No. 301007) AB_314126 (BioLegend Cat. No. 301008) AB_2564167 (BioLegend Cat. No. 301064) AB_2562056 (BioLegend Cat. No. 301051)
- Structure
- Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- Dendritic cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8a on UniProt.org
