Pacific Blue™ anti-human CD8a Antibody
- 产品名称：
- Pacific Blue™ anti-human CD8a Antibody
- 产品类别：
- 抗体
- 产品编号：
- 301026
- 产品应用：
- 301026
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Chimpanzee, Baboon, Pigtailed Macaque, Sooty Mangabey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
?g sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. - Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- ?g sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 5 ?l per 106 cells in 100 ?l volume.
For ?g sizes, the suggested use of this reagent for immunofluorescent staining is ≤1.0 ?g per 106 cells in 100 ?l volume.
It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections3 and costimulation of T cell responses4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
- Magidovich E, et al. 2007. P. Natl. Acad. Sci. USA 104:13022.
- Thakarl D, et al. 2008.J. immunol. 180:7431. PubMed
- Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Product Citations
-
- Toepfner N et al. 2018. eLife. 7 pii: e29213. PubMed
- Tocheva AS, et al. 2020. Curr Protoc Immunol. 130:e103. PubMed
- Petit N, et al. 2015. PLoS One. 10: 0138420. PubMed
- Durgeau A, et al. 2018. Nat Commun. 9:5097. PubMed
- Wu HL, et al. 2018. J Immunol. 200:49. PubMed
- Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
- B?cker-Koduah P, et al. 2020. Ann Clin Transl Neurol. 1.422222222. PubMed
- Koreth J, et al. 2016. Blood. 128: 130 - 137. PubMed
- Stunnenberg M, et al. 2020. Viruses. 12:00. PubMed
- Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed
- Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
- Corgnac S, et al. 2021. STAR Protocols. 2(1):100267. PubMed
- Khazen R, et al. 2016. Nat Commun. 7:10823. PubMed
- RRID
- AB_493111 (BioLegend Cat. No. 301026) AB_1595443 (BioLegend Cat. No. 301033) AB_493110 (BioLegend Cat. No. 301023)
- Structure
- Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- Dendritic cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8a on UniProt.org
