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Brilliant Violet 510™ anti-human CD8a Antibo
产品名称：
Brilliant Violet 510™ anti-human CD8a Antibo
产品类别：
抗体
产品编号：
301047
产品应用：
301047
[价格]
规格 价格 库存
25tests ￥ 2464 1

产品详情

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
Chimpanzee, Baboon, Pigtailed Macaque, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510? under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 510? excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections3 and costimulation of T cell responses4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  4. Magidovich E, et al. 2007. P. Natl. Acad. Sci. USA 104:13022.
  5. Thakarl D, et al. 2008.J. immunol. 180:7431. PubMed
  6. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  7. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  10. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
Product Citations
  1. Wheatley A, et al. 2016. Sci Rep. 6: 26478. PubMed
  2. Townsley SM, et al. 2021. Cell Host Microbe. 29(4):564-578.e9. PubMed
  3. Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed
  4. Eslamizar L, et al. 2021. NPJ Vaccines. 6:15. PubMed
  5. Beraud C, et al. 2018. Virol J. 15:35. PubMed
  6. Nakano M, et al. 2021. Front Immunol. 12:713225. PubMed
  7. Mukhamedova M, et al. 2021. Immunity. 54(4):769-780.e6. PubMed
  8. Ma KY, et al. 2021. Nat Immunol. 22:1590. PubMed
  9. Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed
  10. Cho JW, et al. 2021. Nat Commun. 12:6068. PubMed
  11. Juno JA, et al. 2020. Nat Med. 26:1428. PubMed
  12. Haruna M, et al. 2020. Biol Pharm Bull. 43:399. PubMed
  13. Chandele A, et al. 2016. J Virol. 90(24):11259-11278. PubMed
  14. Aliu B, et al. 2020. Journal of Neurochemistry. 154(5):486-501. PubMed
  15. Liisborg C, et al. 2022. PLoS One. 17:e0269960. PubMed
  16. Wheatley AK, et al. 2021. Cell Rep. 37:109822. PubMed
  17. Tan J, et al. 2021. Sci Transl Med. 13: . PubMed
  18. Nguyen THO, et al. 2021. Immunity. 54:1066. PubMed
  19. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  20. Punik J, et al. 2021. Cell Reports. 35(13):109320. PubMed
  21. Telford W, et al. 2017. Cytometry A. 91:314. PubMed
  22. Lu Y, et al. 2021. Gastroenterology. 161:575. PubMed
  23. Keeton R, et al. 2021. Cell Host Microbe. 29:1611. PubMed
  24. Hammitzsch A, et al. 2015. Proc Natl Acad Sci U S A. 112: 10768-10773. PubMed
  25. Severson J, et al. 2015. Cancer Immunol Res. 3:620. PubMed
  26. Silver J, et al. 2020. Methods Mol Biol. 2121:115. PubMed
  27. Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed
  28. Yang L, et al. 2020. Genes Dis. 7:128. PubMed
  29. Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed
  30. Litchfield K, et al. 2021. Cell. 184(3):596-614.e14. PubMed
  31. Port JR, et al. 2020. J Virol. 94:. PubMed
  32. Kariminia A, et al. 2016. Blood. 127: 3082 - 3091. PubMed
  33. Moguche AO et al. 2017. Cell host & microbe. 21(6):695-706 . PubMed
  34. Townsley SM, et al. 2021. STAR Protocols. 2(3):100771. PubMed
  35. Wang L, et al. 2021. Science. 373:. PubMed
  36. Moreno-Fernandez ME, et al. 2021. STAR Protoc. 2:100937. PubMed
  37. Fong L, et al. 2019. Cancer Discov. 10:40. PubMed
  38. Carnevale G,et al. 2017. Sci Rep.. 10.1038/s41598-017-14838-3. PubMed
  39. Collinson–Pautz MR, et al. 2019. Leukemia. 33:2195. PubMed
  40. Duhen R, et al. 2021. Nat Commun. 12:1047. PubMed
  41. Bruno L, et al. 2016. Immunol Cell Biol. 10.1038/icb.2016.49. PubMed
  42. Mothe B, et al. 2020. Front Immunol. 1.029861111. PubMed
  43. Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
RRID
AB_2561378 (BioLegend Cat. No. 301047) AB_2561942 (BioLegend Cat. No. 301048)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
Distribution

Majority of thymocytes, T cell subset, NK cells

Function
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor
MHC Class I molecules
Cell Type
Dendritic cells, NK cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
925 View all products for this Gene ID
UniProt
View information about CD8a on UniProt.org

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