Biotin anti-human CD8a Antibody
- 产品名称：
- Biotin anti-human CD8a Antibody
- 产品类别：
- 抗体
- 产品编号：
- 301003
- 产品应用：
- 301003
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Chimpanzee, Baboon, Pigtailed Macaque, Sooty Mangabey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections3 and costimulation of T cell responses4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
- Magidovich E, et al. 2007. P. Natl. Acad. Sci. USA 104:13022.
- Thakarl D, et al. 2008.J. immunol. 180:7431. PubMed
- Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Product Citations
-
- Garcia–Perez JE, et al. 2019. Front Immunol. 10:998. PubMed
- Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
- RRID
- AB_314121 (BioLegend Cat. No. 301003) AB_314122 (BioLegend Cat. No. 301004)
- Structure
- Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- Dendritic cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8a on UniProt.org
