APC/Cyanine7 anti-human CD8a Antibody
- 产品名称：
- APC/Cyanine7 anti-human CD8a Antibody
- 产品类别：
- 抗体
- 产品编号：
- 301015
- 产品应用：
- 301015
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2002 |
1 |
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Chimpanzee, Baboon, Pigtailed Macaque, Sooty Mangabey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The CD8a antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections3 and costimulation of T cell responses4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).
- Additional Product Notes
- BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
- Magidovich E, et al. 2007. P. Natl. Acad. Sci. USA 104:13022.
- Thakarl D, et al. 2008.J. immunol. 180:7431. PubMed
- Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Product Citations
-
- Tew G, et al. 2016. Gastroenterology. 150:477-487. PubMed
- Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
- Chiu Y, et al. 2016. Sci Rep. 6:19227. PubMed
- Kimura I, et al. 2022. Cell Rep. 38:110218. PubMed
- Ramien C, et al. 2020. Cell Reports. 29(4):810-815. PubMed
- Sangare K, et al. 2022. Vaccines (Basel). 10:. PubMed
- Woolsey C, et al. 2020. Scientific Reports. 10(1):3071.. PubMed
- Stuart T, et al. 2019. Cell. 177:1888. PubMed
- Jankeel A, et al. 2020. J Virol. :94. PubMed
- Fang F, et al. 2022. JCI Insight. 7:. PubMed
- Spencer A, et al. 2014. PLoS One. 9:100538. PubMed
- Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
- Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
- Natale MA, et al. 2021. PLoS Negl Trop Dis. 15:e0009473. PubMed
- Weinfurter J, et al. 2011. PLoS One. 6:e1002381. PubMed
- Usmani SM et al. 2019. Cell host & microbe. 25(1):73-86 . PubMed
- Mastrogiovanni M, et al. 2022. Sci Adv. 8:eabl5942. PubMed
- Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
- Motozono C, et al. 2021. Cell Host Microbe. . PubMed
- Xue J, et al. 2013. PLoS One. 8:72295. PubMed
- Yuan Z, et al. 2016. J Virol. 90: 7728 - 7739. PubMed
- Gustafson CE, et al. 2020. JCI Insight. 5:. PubMed
- Ruth JH, et al. 2021. JCI Insight. 6:e145662. PubMed
- Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
- Brunk F, et al. 2021. Eur J Immunol. 51:2651. PubMed
- Gamradt S, et al. 2021. iScience. 24:103312. PubMed
- RRID
- AB_314133 (BioLegend Cat. No. 301015) AB_314134 (BioLegend Cat. No. 301016)
- Structure
- Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- Dendritic cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8a on UniProt.org
