APC anti-human CD8a Antibody
- 产品名称：
- APC anti-human CD8a Antibody
- 产品类别：
- 抗体
- 产品编号：
- 301014
- 产品应用：
- 301014
[价格]
| 规格 |
价格 |
库存 |
| 100tests |
￥ 1008 |
1 |
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Chimpanzee, Baboon, Pigtailed Macaque, Sooty Mangabey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections3 and costimulation of T cell responses4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
- Magidovich E, et al. 2007. P. Natl. Acad. Sci. USA 104:13022.
- Thakarl D, et al. 2008.J. immunol. 180:7431. PubMed
- Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Product Citations
-
- Frazzette N, et al. 2020. NPJ Precis Oncol. 4:13. PubMed
- Xiao C, et al. 2022. iScience. 25:103934. PubMed
- Wang Z, et al. 2021. Cell Mol Immunol. 18:2188. PubMed
- Vignard V, et al. 2020. Cancer Immunol Res. 0.510416667. PubMed
- Eriksson E, et al. 2016. Gene Ther. 10.1038/gt.2016.80. PubMed
- Hu W, et al. 2021. Vaccines (Basel). 9:. PubMed
- Vanuytsel K, et al. 2022. Nat Commun. 13:1103. PubMed
- Zhang J, et al. 2022. Nature. 609:369. PubMed
- Cai J, et al. 2021. eLife. 10:00. PubMed
- Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed
- Sperk M, et al. 2021. iScience. 24:102111. PubMed
- Nakasone H, et al. 2021. Commun Biol. 4:1177. PubMed
- Vikkurthi R, et al. 2022. Nat Microbiol. 7:974. PubMed
- Li N, et al. 2020. Oncoimmunology. 9:1824643. PubMed
- Trinklein ND, et al. 2019. MAbs. 11:639. PubMed
- Zheng Q, et al. 2017. Mol Oncol. 10.1002/1878-0261.12056. PubMed
- Hwang B, et al. 2021. Nat Methods. 18:903. PubMed
- Sam J, et al. 2020. Front Oncol. 10:575737. PubMed
- Halim TYF et al. 2018. Immunity. 48(6):1195-1207 . PubMed
- G?rtner K, et al. 2019. J Extracell Vesicles. 8:1573051. PubMed
- ?yszkiewicz M, et al. 2020. Nat Commun. 11:1031. PubMed
- Akusj?rvi SS, et al. 2022. iScience. 25:103607. PubMed
- Corgnac S, et al. 2022. J Immunother Cancer. 10:. PubMed
- Zhai Y, et al. 2018. Autophagy. 1.714583333. PubMed
- Frazzette N, et al. 2020. NPJ Precis Oncol. 4:13. PubMed
- Svensson Akusj?rvi S, et al. 2022. Commun Biol. 5:357. PubMed
- Chang ZL, et al. 2020. Nature Protocols. 15(4):1507-1524.. PubMed
- Cimen Bozkus C, et al. 2021. STAR Protoc. 2:100758. PubMed
- RRID
- AB_314132 (BioLegend Cat. No. 301014) AB_2562054 (BioLegend Cat. No. 301049)
- Structure
- Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- Dendritic cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8a on UniProt.org
