Alexa Fluor® 700 anti-human CD8a Antibody
- 产品名称：
- Alexa Fluor® 700 anti-human CD8a Antibody
- 产品类别：
- 抗体
- 产品编号：
- 301027
- 产品应用：
- 301027
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Chimpanzee, Baboon, Pigtailed Macaque, Sooty Mangabey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.?The suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections3 and costimulation of T cell responses4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
- Magidovich E, et al. 2007. P. Natl. Acad. Sci. USA 104:13022.
- Thakarl D, et al. 2008.J. immunol. 180:7431. PubMed
- Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Product Citations
-
- Carre C, et al. 2021. iScience. 24:102970. PubMed
- Freeman ZT, et al. 2020. J Clin Invest. 130:1405. PubMed
- Nielsen CM, et al. 2021. Cell Reports Medicine. 2(3):100207. PubMed
- Swadling L, et al. 2020. Cell Rep. 30:687. PubMed
- Oostindie SC, et al. 2022. Nat Biotechnol. :. PubMed
- Chandran A, et al. 2022. Cell Rep Med. 3:100557. PubMed
- Iwabuchi R, et al. 2021. Frontiers in Immunology. 12:643040. PubMed
- Gatla H, et al. 2022. Front Med Technol. 4:850565. PubMed
- Vanshylla K, et al. 2022. Cell Host Microbe. 30:69. PubMed
- Boland J, et al. 2014. BMJ Support Palliat Care. 4:357. PubMed
- Pagel J, et al. 2020. Front Immunol. 11:565257. PubMed
- Vadrevu KM, et al. 2022. Sci Rep. 12:12038. PubMed
- Lee-Chang C, et al. 2021. J Exp Med. 218:. PubMed
- Ickrath P, et al. 2019. Biomed Rep. 10:119. PubMed
- Katsuyama E, et al. 2020. Cell Reports. 30(1):112-123.e4.. PubMed
- Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
- Ickrath P, et al. 2018. Int J Mol Med. 42:1116. PubMed
- Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed
- RRID
- AB_493744 (BioLegend Cat. No. 301027) AB_493745 (BioLegend Cat. No. 301028)
- Structure
- Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
- Distribution
-
Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- Dendritic cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- UniProt
- View information about CD8a on UniProt.org
