Ultra-LEAF™ Purified anti-human CD4 Antibody-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1540	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
CyTOF? - Verified
Activ, Block, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections^3,4,5, blocking of T cell activation^1,2, and spatial biology (IBEX)^10,11.? This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Application References (PubMed link indicates BioLegend citation)

Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
Deng MC, et al. 1995. Circulation 91:1647. (IHC)
Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
Lan RY, et al. 2006. Hepatology 43:729.
Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed

Product Citations

NULL, et al. 2022. Cell. 185:916. PubMed

RRID

AB_2810426 (BioLegend Cat. No. 300569) AB_2810427 (BioLegend Cat. No. 300570) AB_2810428 (BioLegend Cat. No. 300571) AB_2810429 (BioLegend Cat. No. 300572) AB_2810430 (BioLegend Cat. No. 300573) AB_2810431 (BioLegend Cat. No. 300574)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution: T cell subset, majority of thymocytes, monocytes/macrophages
Function: MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor: MHC class II molecules, HIV gp120, IL-16
Cell Type: Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
Gene ID: 920 View all products for this Gene ID
UniProt: View information about CD4 on UniProt.org

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