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首页 > 抗体蛋白 > 抗体
Purified anti-human CD4 Antibody
产品名称：
Purified anti-human CD4 Antibody
产品类别：
抗体
产品编号：
300501
产品应用：
300501
[价格]
规格 价格 库存
25µg ￥ 336 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF? - Verified
Activ, Block, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11.? This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
  2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
  3. Deng MC, et al. 1995. Circulation 91:1647. (IHC)
  4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  6. Lan RY, et al. 2006. Hepatology 43:729.
  7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  11. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Liu J, et al. 2018. Cell Physiol Biochem. 45:1034. PubMed
  2. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  3. Shahine A, et al. 2019. Nat Commun. 10:56. PubMed
  4. Meckiff BJ, et al. 2019. J Immunol. 203:1276. PubMed
  5. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  6. Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed
  7. Alroqi F, et al. 2017. J Clin Immunol. . 10.1007/s10875-017-0451-1. PubMed
  8. Willing A, et al. 2018. J Immunol. 200:974. PubMed
  9. Campbell GR, et al. 2021. Theranostics. 11:9009. PubMed
  10. Chakhtoura M, et al. 2021. PLoS Pathog. 17:e1009732. PubMed
  11. Mo Y, et al. 2021. STAR Protocols. 2(2):100453. PubMed
  12. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  13. Kessel C, et al. 2017. Arthritis Rheumatol. 69:1480. PubMed
  14. Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
  15. Liu X, et al. 2022. STAR Protoc. 3:101310. PubMed
  16. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  17. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  18. Li H, et al. 2020. Front Immunol. 11:1777. PubMed
  19. Ga?án-Gómez I, et al. 2022. Nat Med. . PubMed
  20. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  21. Cosgrove PR, et al. 2021. Pediatr Res. . PubMed
  22. Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed
  23. Kinosada H, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006120. PubMed
  24. Peng T, et al. 2021. Front Immunol. 12:735643. PubMed
  25. Jeyanathan M, et al. 2022. JCI Insight. 7:. PubMed
  26. Zhao J, et al. 2017. Sci Immunol. 2:eaan5400. PubMed
  27. Jakob M, et al. 2020. Cells. 10:. PubMed
  28. Gee MH, et al. 2018. Cell. 172:549. PubMed
  29. Salazar Y, et al. 2020. J Clin Invest. 3560:130. PubMed
  30. Henrick BM, et al. 2021. Cell. . PubMed
  31. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  32. Levin SD, et al. 2020. Front Immunol. 10:3086. PubMed
  33. Schwabenland M, et al. 2021. Immunity. . PubMed
  34. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
  35. Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
  36. Senosain MF, et al. 2021. Sci Rep. 11:14424. PubMed
  37. Ford ES, et al. 2021. J Clin Invest. 131:. PubMed
  38. Anna F, et al. 2021. J Immunother Cancer. 9:. PubMed
  39. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  40. Lavaert M, et al. 2020. Frontiers in Immunology. 1.610416667. PubMed
  41. Sutherland APR, et al. 2019. Sci Rep. 9:15302. PubMed
  42. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
  43. Ainai A, et al. 2020. Microbiol Immunol. 64:313. PubMed
  44. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  45. Olety B, et al. 2021. Sci Adv. 7:eabj7398. PubMed
  46. Chiou SH, et al. 2021. Immunity. 54:586. PubMed
RRID
AB_314069 (BioLegend Cat. No. 300501) AB_314070 (BioLegend Cat. No. 300502)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

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