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PerCP anti-human CD4 Antibody
产品名称：
PerCP anti-human CD4 Antibody
产品类别：
抗体
产品编号：
300527
产品应用：
300527
[价格]
规格 价格 库存
25tests ￥ 2142 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11.? This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
  2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
  3. Deng MC, et al. 1995. Circulation 91:1647. (IHC)
  4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  6. Lan RY, et al. 2006. Hepatology 43:729.
  7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  11. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Riner D, et al. 2016. PLoS Negl Trop Dis. 10:e0005180. PubMed
  2. Tran TM, et al. 2020. Immunity. 51(4):750-765. PubMed
  3. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
  4. De Hert E, et al. 2022. Int J Mol Sci. 23:. PubMed
  5. Petit N, et al. 2015. PLoS One. 10: 0138420. PubMed
  6. Diao B, et al. 2020. Front Immunol. 1.032638889. PubMed
  7. Rajashekar JK, et al. 2021. Cell Host Microbe. 29(6):904-916.e6. PubMed
  8. Zhao J, et al. 2021. Front Immunol. 12:658420. PubMed
  9. Boyle M, et al. 2015. J Infect Dis. 212: 416-425. PubMed
  10. Bikker A, et al. 2012. Ann Rheum Dis. 71:1027. PubMed
  11. Wouters K, et al. 2017. Sci Rep. 7:42665. PubMed
  12. Vallejo-Gracia A, et al. 2020. Nat Microbiol. 1.002777778. PubMed
  13. Ye H, et al. 2016. Exp Mol Pathol. 101:341-345. PubMed
  14. Nakaya H, et al. 2016. Proc Natl Acad Sci U S A. 113: 1853 - 1858. PubMed
  15. Smolders J, et al. 2010. PLoS One. 5:e15235. PubMed
  16. Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed
  17. Cherneha M, et al. 2021. Viruses. 13:. PubMed
  18. Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed
  19. Verboven K, et al. 2018. Sci Rep. 8:4677. PubMed
  20. Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
  21. FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed
  22. Conti L, et al. 2014. J Immunol. 188:1011. . PubMed
  23. Boehm D et al. 2017. Cell host & microbe. 21(5):569-579 . PubMed
  24. Venugopal G, et al. 2017. PLoS One. . 10.1371/journal.pone.0188138. PubMed
RRID
AB_893327 (BioLegend Cat. No. 300527) AB_893321 (BioLegend Cat. No. 300528)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

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