热线电话: 021-34661275
产品分类/PRODUCT CLASSIFICATION

上海细胞库

人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|

原代细胞

原代细胞| 永生化细胞|

细胞培养

血清| 无血清冻存| 细胞培养试剂盒| 普通培养基| 完全培养基| 细胞污染预防| 细胞污染清除| 细胞培养辅助试剂|

细胞服务

STR鉴定服务| ATCC代购服务|

酶联试剂盒

人ELISA试剂盒| 大鼠ELISA试剂盒| 小鼠ELISA试剂盒| 兔ELISA试剂盒| 猪ELISA试剂盒| 鱼ELISA试剂盒| 牛ELISA试剂盒| 犬ELISA试剂盒| 鸭ELISA试剂盒| 豚鼠ELISA试剂盒| 鸡ELISA试剂盒| 绵羊ELISA试剂盒| 山羊ELISA试剂盒| 仓鼠ELISA试剂盒| 植物ELISA试剂盒| 药残留类试剂盒| 其他ELISA试剂盒|

生化试剂盒

生化试剂盒| 微量法检测盒| 分光法检测盒| 胶体金检测盒|

PCR试剂盒

荧光探针PCR试剂盒| 染料荧光PCR试剂盒| 核酸扩增/纯化| 普通PCR检测试剂盒| 质粒菌种感受态|

抗体蛋白

蛋白多肽| 抗体| 免疫组化| 免疫球蛋白|

试剂耗材

标准试液| 科研试剂| 缓 冲 液| 实验耗材| 高效液相色谱对照品|

首页 > 抗体蛋白 > 抗体
PE/Cyanine7 anti-human CD4 Antibody
产品名称：
PE/Cyanine7 anti-human CD4 Antibody
产品类别：
抗体
产品编号：
300511
产品应用：
300511
[价格]
规格 价格 库存
25tests ￥ 2002 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11.? This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
  2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
  3. Deng MC, et al. 1995. Circulation 91:1647. (IHC)
  4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  6. Lan RY, et al. 2006. Hepatology 43:729.
  7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  11. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Ratnapriya S, et al. 2021. Cell Reports. 36(9):109622. PubMed
  2. Toepfner N et al. 2018. eLife. 7 pii: e29213. PubMed
  3. Li M, et al. 2021. J Clin Invest. 131:. PubMed
  4. Malinarich F, et al. 2015. J Immunol. 194:5174. PubMed
  5. Nesterenko PA, et al. 2021. Cell Rep. 37:110167. PubMed
  6. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  7. Roberts A, et al. 2021. Sci Rep. 11:4030. PubMed
  8. Chung B, et al. 2012. PLoS One. 7:e42011. PubMed
  9. Wu VH, et al. 2020. JCI Insight. 5:00. PubMed
  10. Frazzette N, et al. 2020. NPJ Precis Oncol. 4:13. PubMed
  11. Preglej T, et al. 2020. JCI Insight. 5(4):. PubMed
  12. Yu N, et al. 2018. Genes Immun. 10.1038/s41435-017-0007-7. PubMed
  13. Rochat MA, et al. 2018. Virol J. 15:191. PubMed
  14. Arra A, et al. 2021. Oncoimmunology. 10:1938475. PubMed
  15. Park JA, et al. 2021. J Hematol Oncol. 14:142. PubMed
  16. Romero-Olmedo AJ, et al. 2022. Nat Microbiol. 7:195. PubMed
  17. Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed
  18. Leite NC, et al. 2020. Cell Reports. 32(2):107894.. PubMed
  19. Kim ST, et al. 2022. Nat Commun. 13:1970. PubMed
  20. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  21. Albanese M, et al. 2022. Nat Methods. 19:81. PubMed
  22. Kim D, et al. 2020. Nat Med. 26:236. PubMed
  23. Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed
  24. Chung B, et al. 2015. PLoS One. 10: 0145258. PubMed
  25. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  26. Audigé A, et al. 2017. BMC Immunology. 10.1186/s12865-017-0209-9. PubMed
  27. Brunsvig PF, et al. 2020. Front Immunol. 11:572172. PubMed
  28. Terzioglu G,et al. 2017. Mech Ageing Dev. . 10.1016/j.mad.2017.09.003. PubMed
  29. Masuda N, et al. 2021. BMC Cancer. 21:1269. PubMed
  30. Rousset F, et al. 2018. Mol Ther Nucleic Acids. 14:351. PubMed
  31. Min HK, et al. 2021. Immune Netw. 21:e43. PubMed
  32. Frazzette N, et al. 2020. NPJ Precis Oncol. 4:13. PubMed
  33. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  34. Hassounah NB, et al. 2019. Cancer Immunol Immunother. 68:407. PubMed
  35. Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  36. Nesterenko PA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
RRID
AB_314079 (BioLegend Cat. No. 300511) AB_314080 (BioLegend Cat. No. 300512)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

联系我们

TEL：021-34661275 　点击拨打热线