PE/Cyanine5 anti-human CD4 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1624	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections^3,4,5, blocking of T cell activation^1,2, and spatial biology (IBEX)^10,11.? This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
Deng MC, et al. 1995. Circulation 91:1647. (IHC)
Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
Lan RY, et al. 2006. Hepatology 43:729.
Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed

Product Citations

Burbano YCB, et al. 2019. Rev Soc Bras Med Trop. 52:e20190101. PubMed
Li G, et al. 2019. J Virol. 93:e00233-19. PubMed
O'Regan N, et al. 2014. PLoS Negl Trop Dis. 8:3206. PubMed
Jung J, et al. 2019. Cell Rep. 26:1906. PubMed
Ryba M, et al. 2011. Cytokine. 55:353. PubMed
Bill M, et al. 2018. J Cell Mol Med. 22:2311. PubMed
Rodriguez‐Meira A et al. 2019. Molecular cell. 73(6):1292-1305 . PubMed

RRID

AB_314077 (BioLegend Cat. No. 300509) AB_314078 (BioLegend Cat. No. 300510)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution: T cell subset, majority of thymocytes, monocytes/macrophages
Function: MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor: MHC class II molecules, HIV gp120, IL-16
Cell Type: Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
Gene ID: 920 View all products for this Gene ID
UniProt: View information about CD4 on UniProt.org

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